ArticleJournal of bacteriology2009
Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient in (p)ppGpp synthetase genes, relA, yjbM, and ywaC.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- Analysis of essential genes inJournal of bacteriology · 2025Article
- Sound the (Smaller) Alarm: The Triphosphate Magic Spot Nucleotide pGpp.Infection and immunity · 2023Review
- At the Crossroad of Nucleotide Dynamics and Protein Synthesis in Bacteria.Microbiology and molecular biology reviews : MMBR · 2023Review
- Bacterial transcription during growth arrest.Transcription · 2021Review
- Effects of DNA Topology on Transcription from rRNA Promoters inMicroorganisms · 2021Article
- Basal-Level Effects of (p)ppGpp in the Absence of Branched-Chain Amino Acids in Actinobacillus pleuropneumoniae.Journal of bacteriology · 2020Article
- RelZ-Mediated Stress Response inJournal of bacteriology · 2020Article
- Analysis of tRNAF1000Research · 2020Article
- Persistence of Intracellular Bacterial Pathogens-With a Focus on the Metabolic Perspective.Frontiers in cellular and infection microbiology · 2020Review
- Pleiotropic Effects of Bacterial Small Alarmone Synthetases: Underscoring the Dual-Domain Small Alarmone Synthetases inFrontiers in microbiology · 2020Review
- The stringent response and Mycobacterium tuberculosis pathogenesis.Pathogens and disease · 2018Review
- From (p)ppGpp to (pp)pGpp: Characterization of Regulatory Effects of pGpp Synthesized by the Small Alarmone Synthetase of Enterococcus faecalis.Journal of bacteriology · 2015Article
- CodY Regulates SigD Levels and Activity by Binding to Three Sites in the fla/che Operon.Journal of bacteriology · 2015Article
- ComGA-RelA interaction and persistence in the Bacillus subtilis K-state.Molecular microbiology · 2015Article
- Many means to a common end: the intricacies of (p)ppGpp metabolism and its control of bacterial homeostasis.Journal of bacteriology · 2015Review
- RelA inhibits Bacillus subtilis motility and chaining.Journal of bacteriology · 2015Article
- Two small (p)ppGpp synthases in Staphylococcus aureus mediate tolerance against cell envelope stress conditions.Journal of bacteriology · 2014Article
- Essential roles for Mycobacterium tuberculosis Rel beyond the production of (p)ppGpp.Journal of bacteriology · 2013Article
- Basal levels of (p)ppGpp in Enterococcus faecalis: the magic beyond the stringent response.mBio · 2013Article
- The δ subunit of RNA polymerase is required for rapid changes in gene expression and competitive fitness of the cell.Journal of bacteriology · 2013Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Bacillus subtilis a null mutation of the relA gene, whose gene product is involved in the synthesis and/or hydrolysis of (p)ppGpp, causes a growth defect that can be suppressed by mutation(s) of yjbM and/or ywaC coding for small (p)ppGpp synthetases. All 35 suppressor mutations newly isolated were classified into two groups, either yjbM or ywaC, by mapping and sequencing their mutations, suggesting that there are no (p)ppGpp synthetases other than RelA, YjbM, and YwaC in B. subtilis. In order to understand better the relation between RelA and rRNA synthesis, we studied in the relA mutant the transcriptional regulation of seven rRNA operons (rrnO, -A, -J, -I, -E, -D, or -B) individually after integration of a promoter- and terminatorless cat gene. We identified the transcriptional start sites of each rrn operon (a G) and found that transcription of all rrn operons from their P1 promoters was drastically reduced in the relA mutant while this was almost completely restored in the relA yjbM ywaC triple mutant. Taken together with previous results showing that the intracellular GTP concentration was reduced in the relA mutant while it was restored in the triple mutant, it seems likely that continuous (p)ppGpp synthesis by YjbM and/or YwaC at a basal level causes a decrease in the amounts of intracellular GTP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.