ArticleJournal of bacteriology2009
Activation of the promoter of the fengycin synthetase operon by the UP element.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Surfactin-Bacillaene Copathway Engineering Strategy Boosts Fengycin Production and Antifungal Activity inMicroorganisms · 2026Article
- Exploration of genes encoding KEGG pathway enzymes in rhizospheric microbiome of the wild plant Abutilon fruticosum.AMB Express · 2024Article
- Key elements and regulation strategies of NRPSs for biosynthesis of lipopeptides by Bacillus.Applied microbiology and biotechnology · 2020Review
- A repeat sequence causes competition of ColE1-type plasmids.PloS one · 2013Article
- A bile-inducible membrane protein mediates bifidobacterial bile resistance.Microbial biotechnology · 2012Article
- Diversity of nonribosomal peptide synthetases involved in the biosynthesis of lipopeptide biosurfactants.International journal of molecular sciences · 2010Review
- Production and characterization of fengycin by indigenous Bacillus subtilis F29-3 originating from a potato farm.International journal of molecular sciences · 2010Article
- A sequence that affects the copy number and stability of pSW200 and ColE1.Journal of bacteriology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis F29-3 produces an antifungal peptidic antibiotic that is synthesized nonribosomally by fengycin synthetases. Our previous work established that the promoter of the fengycin synthetase operon is located 86 nucleotides upstream of the translational initiation codon of fenC. This investigation involved transcriptional fusions with a DNA fragment that contains the region between positions -105 and +80 and determined that deleting the region between positions -55 and -42 reduces the promoter activity by 64.5%. Transcriptional fusions in the B. subtilis DB2 chromosome also indicated that mutating the sequence markedly reduces the promoter activity. An in vitro transcription analysis confirmed that the transcription is inefficient when the sequence in this region is mutated. Electrophoretic mobility shift and footprinting analyses demonstrated that the C-terminal domain of the RNA polymerase alpha subunit binds to the region between positions -55 and -39. These results indicated that the sequence is an UP element. Finally, this UP element is critical for the production of fengycin, since mutating the UP sequence in the chromosome of B. subtilis F29-3 reduces the transcription of the fen operon by 85% and prevents the cells from producing enough fengycin to suppress the germination of Paecilomyces variotii spores on agar plates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.