ArticleJournal of bacteriology2009
The oligopeptide transport system is essential for the development of natural competence in Streptococcus thermophilus strain LMD-9.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed, 132 citations in OpenAlex.
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- Comparative functional profiles of microbial communities on drifting microplastics and volcanic pumice.ISME communications · 2026Article
- Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes.NPJ biofilms and microbiomes · 2025Article
- Multi-way modelling of oral microbial dynamics and host-microbiome interactions during induced gingivitis.NPJ biofilms and microbiomes · 2024Article
- Use of Rgg quorum-sensing machinery to create an innovative recombinant protein expression system inMicrobiology (Reading, England) · 2024Article
- Genetic modification ofmSphere · 2024Article
- Mechanisms of the Quorum Sensing Systems ofCurrent medicinal chemistry · 2024Review
- The ComRS-SigX Pathway Regulates Natural Transformation in Streptococcus ferus.Journal of bacteriology · 2023Article
- The ComRS-SigX pathway regulates natural transformation inbioRxiv : the preprint server for biology · 2023Article
- Inoculation withFrontiers in microbiology · 2023Article
- Modulating streptococcal phenotypes using signal peptide analogues.Open biology · 2022Review
- Expanding natural transformation to improve beneficial lactic acid bacteria.FEMS microbiology reviews · 2022Article
- Role of the Sortase A in the Release of Cell-Wall Proteinase PrtS in the Growth Medium ofMicroorganisms · 2021Article
- Three Distinct Proteases Are Responsible for Overall Cell Surface Proteolysis in Streptococcus thermophilus.Applied and environmental microbiology · 2021Article
- Identification ofMicroorganisms · 2021Article
- Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans.The ISME journal · 2021Article
- Multi-omics Approach Reveals How Yeast Extract Peptides Shape Streptococcus thermophilus Metabolism.Applied and environmental microbiology · 2020Article
- Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In gram-positive bacteria, oligopeptide transport systems, called Opp or Ami, play a role in nutrition but are also involved in the internalization of signaling peptides that take part in the functioning of quorum-sensing pathways. Our objective was to reveal functions that are controlled by Ami via quorum-sensing mechanisms in Streptococcus thermophilus, a nonpathogenic bacterium widely used in dairy technology in association with other bacteria. Using a label-free proteomic approach combining one-dimensional electrophoresis with liquid chromatography-tandem mass spectrometry analysis, we compared the proteome of the S. thermophilus LMD-9 to that of a mutant deleted for the subunits C, D, and E of the ami operon. Both strains were grown in a chemically defined medium (CDM) without peptides. We focused our attention on proteins that were no more detected in the ami deletion mutant. In addition to the three subunits of the Ami transporter, 17 proteins fulfilled this criterion and, among them, 7 were similar to proteins that have been identified as essential for transformation in S. pneumoniae. These results led us to find a condition of growth, the early exponential state in CDM, that allows natural transformation in S. thermophilus LMD-9 to turn on spontaneously. Cells were not competent in M17 rich medium. Furthermore, we demonstrated that the Ami transporter controls the triggering of the competence state through the control of the transcription of comX, itself controlling the transcription of late competence genes. We also showed that one of the two oligopeptide-binding proteins of strain LMD-9 plays the predominant role in the control of competence.
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