ArticleThe Journal of biological chemistry2009
Intestinal anion exchanger down-regulated in adenoma (DRA) is inhibited by intracellular calcium.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Spironolactone increases serum uric acid levels in patients with chronic kidney disease.Journal of human hypertension · 2014Trial
- Multiple Regulatory Signals and Components in the Modulation of Bicarbonate Transporters.Pharmaceutics · 2024Review
- SLC26 Anion Transporters.Handbook of experimental pharmacology · 2024Review
- SLC26A3 (DRA) is stimulated in a synergistic, intracellular CaAmerican journal of physiology. Cell physiology · 2023Article
- Advances in CaExperimental and therapeutic medicine · 2020Review
- Loss of MYO5B Leads to Reductions in NaGastroenterology · 2018Article
- Keratin 8 knockdown leads to loss of the chloride transporter DRA in the colon.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Bile acids inhibit Na⁺/H⁺ exchanger and Cl⁻/HCO₃⁻ exchanger activities via cellular energy breakdown and Ca²⁺ overload in human colonic crypts.Pflugers Archiv : European journal of physiology · 2015Article
- Congenital chloride-losing diarrhea in a Mexican child with the novel homozygous SLC26A3 mutation G393W.Frontiers in physiology · 2015Article
- Functional regulation of the SLC26-family protein prestin by calcium/calmodulin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Translating molecular physiology of intestinal transport into pharmacologic treatment of diarrhea: stimulation of Na+ absorption.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2014Review
- Article
- Regulation of electroneutral NaCl absorption by the small intestine.Annual review of physiology · 2011Review
- Alterations in the proteome of the NHERF1 knockout mouse jejunal brush border membrane vesicles.Physiological genomics · 2010Article
- Activity and PI3-kinase dependent trafficking of the intestinal anion exchanger downregulated in adenoma depend on its PDZ interaction and on lipid rafts.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
- Review
- The SLC26 gene family of anion transporters and channels.Molecular aspects of medicineReview
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Na/H exchanger 3 (NHE3) and the Cl/HCO(3) exchanger down-regulated in adenoma (DRA) together facilitate intestinal electroneutral NaCl absorption. Elevated Ca(2+)(i) inhibits NHE3 through mechanisms involving the PDZ domain proteins NHE3 kinase A regulatory protein (E3KARP) or PDZ kidney 1 (PDZK1). DRA also possesses a PDZ-binding motif, but the roles of interactions with E3KARP or PDZK1 and Ca(2+)(i) in DRA regulation are unknown. Wild type DRA and a mutant lacking the PDZ interaction motif (DRA-ETKFminus) were expressed constitutively in human embryonic kidney (HEK) and inducibly in Caco-2/BBE cells. DRA-mediated Cl/HCO(3) exchange was measured as intracellular pH changes. Ca(2+)(i) was assessed fluorometrically. DRA was induced 8-16-fold and was delivered to the apical surface of polarized Caco-2 cells. Putative anion transporter 1 and cystic fibrosis transmembrane regulator did not contribute to Cl/HCO(3) exchange in transfected Caco-2 cells. The calcium ionophore 4Br-A23187 inhibited DRA and DRA-ETKFminus in HEK cells, but only full-length DRA was inhibited in Caco-2 cells. In contrast, 100 microm UTP, which increased Ca(2+)(i), inhibited full-length DRA but not DRA-ETKFminus in Caco-2 and HEK cells. In HEK cells, which express little PDZK1, additional transfection of PDZK1 was required for UTP to inhibit DRA. As HEK cells do not express cystic fibrosis transmembrane regulator or NHE3, the data indicate that Ca(2+)(i)-dependent DRA inhibition is not because of modulation of other transport activities. In polarized epithelium, this inhibition requires interaction of DRA with PDZK1. Together with data from PDZK1(-/-) mice, these data underscore the prominent role of PDZK1 in Ca(2+)(i)-mediated inhibition of colonic NaCl absorption.
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