ArticleThyroid : official journal of the American Thyroid Association2009
Comparison of thyroidectomized calf serum and stripped serum for the study of thyroid hormone action in human skin fibroblasts in vitro.
Article in Thyroid : official journal of the American Thyroid Association, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.
- American Thyroid Association Guide to investigating thyroid hormone economy and action in rodent and cell models.Thyroid : official journal of the American Thyroid Association · 2014Guideline
- Roxadustat Activates Thyroid Hormone Receptors α and β at Clinically Relevant Concentrations.Cureus · 2025Article
- Impaired T3 uptake and action in MCT8-deficient cerebral organoids underlie Allan-Herndon-Dudley syndrome.JCI insight · 2024Article
- The Differential Effect of a Shortage of Thyroid Hormone Compared with Knockout of Thyroid Hormone Transporters Mct8 and Mct10 on Murine Macrophage Polarization.International journal of molecular sciences · 2024Article
- Cell-Specific Transport and Thyroid Hormone Receptor Isoform Selectivity Account for Hepatocyte-Targeted Thyromimetic Action of MGL-3196.International journal of molecular sciences · 2022Article
- Higher Thyroid-Stimulating Hormone, Triiodothyronine and Thyroxine Values Are Associated with Better Outcome in Acute Liver Failure.PloS one · 2015Article
- Thyroid hormone receptor α and regulation of type 3 deiodinase.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Transcriptional profiling of fibroblasts from patients with mutations in MCT8 and comparative analysis with the human brain transcriptome.Human molecular genetics · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundKnowledge on the action of thyroid hormone (TH) is augmented by the study of tissue responses to TH in vitro. In order to support the growth of cells in vitro, calf serum (CS) is usually added to the medium to provide necessary nutrients and growth factors. However, the content of endogenous TH in the CS may obfuscate changes with small doses of TH. We therefore compared the use of TH-depleted medium, either by resin treatment (stripped-CS) or by the use of CS from a thyroidectomized calf (TxCS) for gene expression studies.
methodsWe describe the method for preparing a thyroidectomized calf, harvesting the blood and preparing the serum. We utilized methimazole in conjunction with the thyroidectomy to prevent TH synthesis in the event of regrowth of the thyroid remnant.
resultsTotal triiodothyronine (T(3)) and thyroxine concentrations in TxCS were low at <30 ng/dL and <1 microg/dL, respectively. We compared the effect of T(3) on basic transcription element-binding protein (BTEB)1 and stanniocalcin (STC)-1 mRNA expression in human fibroblasts from a normal individual and a subject with resistance to TH (RTH) cultured in stripped-CS to TxCS and demonstrated that with stripped-CS and TxCS differences in the BTEB1 and STC-1 expression of normal and RTH fibroblasts could be detected.
conclusionsBoth stripped-CS and TxCS are suitable to detect subtle differences in TH responsiveness between normal and RTH human skin fibroblasts, yet TxCS is not as costly as stripped-CS and relatively easy to prepare.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.