Evidence map›Paper›PMID 19444338›Full record

ArticleIEEE journal of quantum electronics2005

Ultrafast Method for the Analysis of Fluorescence Lifetime Imaging Microscopy Data Based on the Laguerre Expansion Technique.

Javier A Jo, Qiyin Fang, Laura Marcu

Abstract read
In one paragraph

Article in IEEE journal of quantum electronics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
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  4. Review
  5. Article
  6. Visualization of Nanocarriers and Drugs in Cells and Tissue.Handbook of experimental pharmacology · 2024
    Article
  7. Real-time open-source FLIM analysis.Frontiers in bioinformatics · 2023
    Article
  8. Article
  9. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Javier A JoJ. A. Jo is with the Biophotonics Research and Technology Development, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048 USA (e-mail: joj@cshs.org ).
Qiyin Fang
Laura Marcu

Funding

Time-Resolved Spectroscopy of Unstable Arterial PlaquesR01HL067377 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MARCU, LAURA · 2001 to 2015
$5.2M
NHLBI NIH HHS R01 HL067377
6 · The paper itself

Abstract

We report a new deconvolution method for fluorescence lifetime imaging microscopy (FLIM) based on the Laguerre expansion technique. The performance of this method was tested on synthetic and real FLIM images. The following interesting properties of this technique were demonstrated. 1) The fluorescence intensity decay can be estimated simultaneously for all pixels, without a priori assumption of the decay functional form. 2) The computation speed is extremely fast, performing at least two orders of magnitude faster than current algorithms. 3) The estimated maps of Laguerre expansion coefficients provide a new domain for representing FLIM information. 4) The number of images required for the analysis is relatively small, allowing reduction of the acquisition time. These findings indicate that the developed Laguerre expansion technique for FLIM analysis represents a robust and extremely fast deconvolution method that enables practical applications of FLIM in medicine, biology, biochemistry, and chemistry.

Identifiers

PMID19444338
PMCPMC2681327

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.