ArticleNature protocols2009
A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS).
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Targeted Proteomics Reveals Quantitative Differences in Low-Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation.International journal of molecular sciences · 2024Article
- Spliceosomal Prp8 intein at the crossroads of protein and RNA splicing.PLoS biology · 2019Article
- Acquired cross-linker resistance associated with a novel spliced BRCA2 protein variant for molecular phenotyping of BRCA2 disruption.Cell death & disease · 2017Article
- Tyrosine-Nitrated Proteins: Proteomic and Bioanalytical Aspects.Antioxidants & redox signaling · 2017Review
- Dorsal root ganglia in Friedreich ataxia: satellite cell proliferation and inflammation.Acta neuropathologica communications · 2016Article
- FLEXITau: Quantifying Post-translational Modifications of Tau Protein in Vitro and in Human Disease.Analytical chemistry · 2016Article
- A Review of the Effects of Major Atmospheric Pollutants on Pollen Grains, Pollen Content, and Allergenicity.TheScientificWorldJournal · 2015Review
- Article
- Unblocking the sink: improved CID-based analysis of phosphorylated peptides by enzymatic removal of the basic C-terminal residue.Journal of the American Society for Mass Spectrometry · 2014Article
- Drosophila F-BAR protein Syndapin contributes to coupling the plasma membrane and contractile ring in cytokinesis.Open biology · 2013Article
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- Review of software tools for design and analysis of large scale MRM proteomic datasets.Methods (San Diego, Calif.) · 2013Review
- Capillary zone electrophoresis-multiple reaction monitoring from 100 pg of RAW 264.7 cell lysate digest.The Analyst · 2013Article
- Article
- Histaminylation of glutamine residues is a novel posttranslational modification implicated in G-protein signaling.FEBS letters · 2012Article
- Pyruvate kinase is a dosage-dependent regulator of cellular amino acid homeostasis.Oncotarget · 2012Article
- The dormancy regulator DosR controls ribosome stability in hypoxic mycobacteria.The Journal of biological chemistry · 2012Article
- Advancing the sensitivity of selected reaction monitoring-based targeted quantitative proteomics.Proteomics · 2012Review
- Mapping protein interactions by combining antibody affinity maturation and mass spectrometry.Analytical biochemistry · 2011Article
- Selected reaction monitoring mass spectrometry reveals the dynamics of signaling through the GRB2 adaptor.Nature biotechnology · 2011Article
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Authors and funding
3 authors.
Funding
Abstract
The application of a targeted mass spectrometric workflow to the sensitive identification of post-translational modifications is described. This protocol employs multiple reaction monitoring (MRM) to search for all putative peptides specifically modified in a target protein. Positive MRMs trigger an MS/MS experiment to confirm the nature and site of the modification. This approach, termed MIDAS (MRM-initiated detection and sequencing), is more sensitive than approaches using neutral loss scanning or precursor ion scanning methodologies, due to a more efficient use of duty cycle along with a decreased background signal associated with MRM. We describe the use of MIDAS for the identification of phosphorylation, with a typical experiment taking just a couple of hours from obtaining a peptide sample. With minor modifications, the MIDAS method can be applied to other protein modifications or unmodified peptides can be used as a MIDAS target.
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