ArticleNature protocols2009
Targeted single-cell electroporation of mammalian neurons in vivo.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 128 citations in OpenAlex.
- Voltage dynamics of cortical dendrites in vivo.Nature neuroscience · 2026Article
- Electroporation of Whole-Mount Postnatal Rodent Retinas for Advanced Functional Assays.Bio-protocol · 2026Article
- Live STED imaging of functional neuroanatomy.Nature protocols · 2025Review
- Visually guided in vivo single-cell electroporation for monitoring and manipulating mammalian hippocampal neurons.Nature protocols · 2025Review
- Article
- DeepD3, an open framework for automated quantification of dendritic spines.PLoS computational biology · 2024Article
- Daily two-photon neuronal population imaging with targeted single-cell electrophysiology and subcellular imaging in auditory cortex of behaving mice.Frontiers in cellular neuroscience · 2023Article
- All-atom molecular dynamics simulations of the combined effects of different phospholipids and cholesterol content on electroporation.RSC advances · 2022Article
- Laser Machined Fiber-based Microprobe: Application in Microscale Electroporation.Advanced fiber materials · 2022Article
- Brain-wide projection reconstruction of single functionally defined neurons.Nature communications · 2022Article
- Sample Preparation and Warping Accuracy for Correlative Multimodal Imaging in the Mouse Olfactory Bulb Using 2-Photon, Synchrotron X-Ray and Volume Electron Microscopy.Frontiers in cell and developmental biology · 2022Article
- Visualizing the Intracellular Trafficking in Zebrafish Mauthner Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- PEGylation of mRNA by Hybridization of Complementary PEG-RNA Oligonucleotides Stabilizes mRNA without Using Cationic Materials.Pharmaceutics · 2021Article
- Axonal and Dendritic Morphology of Excitatory Neurons in Layer 2/3 Mouse Barrel Cortex Imaged Through Whole-Brain Two-Photon Tomography and Registered to a Digital Brain Atlas.Frontiers in neuroanatomy · 2021Article
- Article
- Article
- Single-Cell Visualization Deep in Brain Structures by Gene Transfer.Frontiers in neural circuits · 2020Article
- Article
- Wearable Devices for Single-Cell Sensing and Transfection.Trends in biotechnology · 2019Review
- Magnetically guided virus stamping for the targeted infection of single cells or groups of cells.Nature protocols · 2019Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
In order to link our knowledge of single neurons with theories of network function, it has been a long-standing goal to manipulate the activity and gene expression of identified subsets of mammalian neurons within the intact brain in vivo. This protocol describes a method for delivering plasmid DNA into single identified mammalian neurons in vivo, by combining two-photon imaging with single-cell electroporation. Surgery, mounting of a chronic recording chamber and targeted electroporation of identified neurons can be performed within 1-2 h. Stable transgene expression can reliably be induced with high success rates both in single neurons as well as in small, spatially defined networks of neurons in the cerebral cortex of rodents.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.