ArticleMolecular & cellular proteomics : MCP2009
An integrated transcriptomics and proteomics analysis of the secretome of the helminth pathogen Fasciola hepatica: proteins associated with invasion and infection of the mammalian host.
Article in Molecular & cellular proteomics : MCP, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.
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Who cites it
144 citing papers in PubMed, 267 citations in OpenAlex.
- Single-cell transcriptomics identifies a p21-activated kinase important for survival of the zoonotic parasiteiScience · 2026Article
- Proteomic characterization of adult Fasciola gigantica recovered from naturally infected buffaloes in Punjab Pakistan.PloS one · 2026Article
- First analysis of Spirometra mansoni excretory-secretory proteins by the 4D-DIA method.Parasite (Paris, France) · 2026Article
- The Calicophoron daubneyi genome provides new insight into mechanisms of feeding, eggshell synthesis and parasite-microbe interactions.BMC biology · 2025Article
- Distinct gut microbiome features characterizeFrontiers in cellular and infection microbiology · 2025Article
- Immune modulation of buffalo peripheral blood mononuclear cells by two asparaginyl endopeptidases from Fasciola gigantica.Parasites & vectors · 2024Article
- A Comprehensive Multi-Omics Study of Serum Alterations in Red Deer Infected by the Liver FlukePathogens (Basel, Switzerland) · 2024Article
- Long noncoding RNA MALAT-1: A versatile regulator in cancer progression, metastasis, immunity, and therapeutic resistance.Non-coding RNA research · 2024Review
- Prostaglandin synthase activity of sigma- and mu-class glutathione transferases in a parasitic trematode, Clonorchis sinensis.Parasites, hosts and diseases · 2024Article
- Trematode Genomics and Proteomics.Advances in experimental medicine and biology · 2024Article
- Glycan Complexity and Heterogeneity of Glycoproteins in Somatic Extracts and Secretome of the Infective Stage of the Helminth Fasciola hepatica.Molecular & cellular proteomics : MCP · 2023Article
- Molecular Characterization of the Interplay betweenInternational journal of molecular sciences · 2023Article
- Identification and genetic characterisatin of cathepsin L in Demodex.Experimental & applied acarology · 2023Article
- Fasciola hepatica juveniles interact with the host fibrinolytic system as a potential early-stage invasion mechanism.PLoS neglected tropical diseases · 2023Article
- Liver Proteome Alterations in Red Deer (Pathogens (Basel, Switzerland) · 2022Article
- Xenobiotic-Metabolizing Enzymes in Trematodes.Biomedicines · 2022Review
- Transcriptomic analysis supports a role for the nervous system in regulating growth and development of Fasciola hepatica juveniles.PLoS neglected tropical diseases · 2022Article
- Proteomics coupled with in vitro model to study the early crosstalk occurring between newly excysted juveniles of Fasciola hepatica and host intestinal cells.PLoS neglected tropical diseases · 2022Article
- Study of the migration of Fasciola hepatica juveniles across the intestinal barrier of the host by quantitative proteomics in an ex vivo model.PLoS neglected tropical diseases · 2022Article
- Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host's body temperature.Scientific reports · 2022Article
84 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
To infect their mammalian hosts, Fasciola hepatica larvae must penetrate and traverse the intestinal wall of the duodenum, move through the peritoneum, and penetrate the liver. After migrating through and feeding on the liver, causing extensive tissue damage, the parasites move to their final niche in the bile ducts where they mature and produce eggs. Here we integrated a transcriptomics and proteomics approach to profile Fasciola secretory proteins that are involved in host-pathogen interactions and to correlate changes in their expression with the migration of the parasite. Prediction of F. hepatica secretory proteins from 14,031 expressed sequence tags (ESTs) available from the Wellcome Trust Sanger Centre using the semiautomated EST2Secretome pipeline showed that the major components of adult parasite secretions are proteolytic enzymes including cathepsin L, cathepsin B, and asparaginyl endopeptidase cysteine proteases as well as novel trypsin-like serine proteases and carboxypeptidases. Proteomics analysis of proteins secreted by infective larvae, immature flukes, and adult F. hepatica showed that these proteases are developmentally regulated and correlate with the passage of the parasite through host tissues and its encounters with different host macromolecules. Proteases such as FhCL3 and cathepsin B have specific functions in larvae activation and intestinal wall penetration, whereas FhCL1, FhCL2, and FhCL5 are required for liver penetration and tissue and blood feeding. Besides proteases, the parasites secrete an array of antioxidants that are also highly regulated according to their migration through host tissues. However, whereas the proteases of F. hepatica are secreted into the parasite gut via a classical endoplasmic reticulum/Golgi pathway, we speculate that the antioxidants, which all lack a signal sequence, are released via a non-classical trans-tegumental pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.