ArticleMethods (San Diego, Calif.)2009
Cost-effective sequence-based nonhuman primate MHC class I genotyping from RNA.
Article in Methods (San Diego, Calif.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Combination therapy with broadly neutralizing antibodies, antiretroviral therapy and CCR5 blockade limits viral reservoir seeding in infant macaque model of HIV.Nature microbiology · 2026Article
- Next-Generation Short-Read Sequencing Reveals Impacts on Major Histocompatibility Complex Diversity Resulting From Differences in Captive Rhesus Macaque (Macaca Mulatta) Colony Expansion Strategies.American journal of primatology · 2026Article
- Determination of major histocompatibility class I and class II genetic composition of the Caribbean Primate Center specific pathogen-free rhesus macaque (Macaca mulatta) colony based on massively parallel sequencing.Journal of medical primatology · 2018Article
- Discovery of novel MHC-class I alleles and haplotypes in Filipino cynomolgus macaques (Macaca fascicularis) by pyrosequencing and Sanger sequencing: Mafa-class I polymorphism.Immunogenetics · 2015Article
- Survey of major histocompatibility complex class II diversity in pig-tailed macaques.Immunogenetics · 2014Article
- Haplessly hoping: macaque major histocompatibility complex made easy.ILAR journal · 2013Review
- Nonhuman primate transplant models finally evolve: detailed immunogenetic analysis creates new models and strengthens the old.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2012Review
- Immunogenetic Management Software: a new tool for visualization and analysis of complex immunogenetic datasets.Immunogenetics · 2012Article
- Article
- Identification of novel MHC class I sequences in pig-tailed macaques by amplicon pyrosequencing and full-length cDNA cloning and sequencing.Immunogenetics · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The major histocompatibility complex (MHC) class I region of nonhuman primates (NHPs) is highly polymorphic and has undergone complex segmental duplications such that the number of expressed genes differs between individuals. In addition the relative abundance of transcripts varies more than 100-fold between NHP class I genes. This unparalleled complexity makes rapid, efficient class I genotyping difficult for NHPs. The 'gold standard' of cDNA library construction, screening and sequencing is both costly and labor-intensive. Several rapid genotyping methods have been utilized, but all require some degree of prior sequence knowledge. Here, we describe a method for sequence-based MHC class I genotyping which reduces cost by (1) pooling molecularly barcoded class I cDNA-PCR amplicons for cloning and (2) targeting sequencing of a region of concentrated polymorphism spanning the two exons encoding the peptide binding domain. This method can efficiently genotype both known and novel MHC class I alleles. In addition, full-length cDNA amplicons with novel sequences can be resequenced in their entireties to expand the repertoire of characterized MHC class I sequences for NHPs.
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Registered trials
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