ArticleAnalytical biochemistry2009
Quantification of regional DNA methylation by liquid chromatography/tandem mass spectrometry.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Adenosine Kinase Expression Determines DNA Methylation in Cancer Cell Lines.ACS pharmacology & translational science · 2021Article
- Epigenetics for Clinicians from the Perspective of Pediatric Rheumatic Diseases.Current rheumatology reports · 2020Review
- Tracking Decitabine Incorporation into Malignant Myeloid Cell DNA in vitro and in vivo by LC-MS/MS with Enzymatic Digestion.Scientific reports · 2019Article
- Global DNA methylation loss associated with mercury contamination and aging in the American alligator (Alligator mississippiensis).The Science of the total environment · 2016Article
- DNA Methylation Analysis: Choosing the Right Method.Biology · 2016Review
- A Novel [Journal of analytical & bioanalytical techniques · 2015Article
- Mass spectrometry of structurally modified DNA.Chemical reviews · 2013Review
- Curcumin down-regulates DNA methyltransferase 1 and plays an anti-leukemic role in acute myeloid leukemia.PloS one · 2013Article
- Reactivation of RASSF1A in breast cancer cells by curcumin.Nutrition and cancer · 2012Article
- Reduced susceptibility of DNA methyltransferase 1 hypomorphic (Dnmt1N/+) mice to hepatic steatosis upon feeding liquid alcohol diet.PloS one · 2012Article
- A sensitive mass spectrometry method for simultaneous quantification of DNA methylation and hydroxymethylation levels in biological samples.Analytical biochemistry · 2011Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Promoter hypermethylation-associated tumor suppressor gene (TSG) silencing has been explored as a therapeutic target for hypomethylating agents. Promoter methylation change may serve as a pharmacodynamic endpoint for evaluation of the efficacy of these agents and predict the patient's clinical response. Here a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay has been developed for quantitative regional DNA methylation analysis using the molar ratio of 5-methyl-2'-deoxycytidine (5mdC) to 2'-deoxycytidine (2dC) in the enzymatic hydrolysate of fully methylated bisulfite-converted polymerase chain reaction (PCR) amplicons as the methylation indicator. The assay can differentiate 5% of promoter methylation level with an intraday precision ranging from 3 to 16% using two TSGs: HIN-1 and RASSF1A. This method was applied to characterize decitabine-induced promoter DNA methylation changes of these two TSGs in a breast cancer MCF-7 cell line. Promoter methylation of these TSGs was found to decrease in a dose-dependent manner. Correspondingly, the expression of these TSGs was enhanced. The sensitivity and reproducibility of the method make it a valuable tool for specific gene methylation analysis that could aid characterization of hypomethylating activity on specific genes by hypomethylating agents in a clinical setting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.