ArticleNaunyn-Schmiedeberg's archives of pharmacology2009
Suppressive effect of CTB glycoprotein (75 kDa) on IL-4 expression in primary-cultured lymphocytes treated with di(2-ethylhexyl) phthalate.
Article in Naunyn-Schmiedeberg's archives of pharmacology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 13 citations in OpenAlex.
- Article
- The association between phthalate exposure and asthma.The Kaohsiung journal of medical sciences · 2012Review
- IgE, COX-2, and IL-4 are expressed by DEHP through p38 MAPK and suppressed by plant glycoprotein (75 kDa) in ICR mice.Inflammation · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study is to determine the inhibitory effect of a glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein, 75 kDa) on di(2-ethylhexyl) phthalate (DEHP)-induced differentiation of T helper (Th) type 2 cells in T lymphocytes separated from mice. This experiment evaluated the activities of protein kinase C (PKC), mitogen-activated protein kinase (MAPK), transcription factors [signal transducer and activator of transcription (STAT)-6 and GATA-binding protein 3 (GATA3)], and Th2 cell-related cytokine [interleukin-4 (IL-4)] using immunoblotting and reverse transcription-polymerase chain reaction. Our results revealed that the CTB glycoprotein in the presence of DEHP inhibits the translocation of PKC from cytosol to membrane and the phosphorylation of p44/42 MAPK in primary cultured T lymphocytes. We also found that the CTB glycoprotein (100 microg/ml) has suppressive effects on transcriptional activation of STAT6, GATA3, and on the expression level of IL-4 in DEHP-treated T lymphocytes. The phosphorylation of STAT6 and GATA3 were collectively blocked by treatment with PKC inhibitor (staurosporine) as well as p44/42 MAPK inhibitor (PD 98059), respectively. The results from these experiments indicated that the CTB glycoprotein inhibits IL-4 expression, not IL-10 expression, on the stage of Th2 cell differentiation induced by DEHP in T lymphocytes. Hence, we speculate that the CTB glycoprotein has a strong inhibitory ability for expressions of allergy-related cytokines indirectly caused by DEHP in Th2 cell differentiation of the primary cultured mouse T lymphocytes.
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