ArticlePloS one2009
Lipid raft-dependent FcepsilonRI ubiquitination regulates receptor endocytosis through the action of ubiquitin binding adaptors.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- The role of HECT-type E3 ubiquitin ligases in inflammation.Frontiers in immunology · 2026Review
- SCF and IL-33 regulate mouse mast cell phenotypic and functional plasticity supporting a pro-inflammatory microenvironment.Cell death & disease · 2023Article
- Cholesterol in the Cell Membrane-An Emerging Player in Atherogenesis.International journal of molecular sciences · 2022Review
- FcεRI Signaling in the Modulation of Allergic Response: Role of Mast Cell-Derived Exosomes.International journal of molecular sciences · 2020Review
- Direct Inhibition of the Allergic Effector Response by Raw Cow's Milk-An Extensive In Vitro Assessment.Cells · 2020Article
- SLAP Is a Negative Regulator of FcεRI Receptor-Mediated Signaling and Allergic Response.Frontiers in immunology · 2019Article
- Imidacloprid inhibits IgE-mediated RBL-2H3 cell degranulation and passive cutaneous anaphylaxis.Asia Pacific allergy · 2016Article
- Review
- New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling.Molecular and cellular biology · 2016Article
- A Combination of Screening and Computational Approaches for the Identification of Novel Compounds That Decrease Mast Cell Degranulation.Journal of biomolecular screening · 2015Article
- Regulation of fc receptor endocytic trafficking by ubiquitination.Frontiers in immunology · 2014Review
- The architectural relationship of components controlling mast cell endocytosis.Journal of cell science · 2013Article
- The effects of low-dose ionizing radiation in the activated rat basophilic leukemia (RBL-2H3) mast cells.The Journal of biological chemistry · 2012Article
- Lipid rafts in mast cell biology.Journal of lipids · 2011Article
- β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion.Scientific reports · 2011Article
- How ubiquitination and autophagy participate in the regulation of the cell response to bacterial infection.Biology of the cell · 2010Review
- Endocytosis and intracellular trafficking of human natural killer cell receptors.Traffic (Copenhagen, Denmark) · 2009Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The best characterized role for ubiquitination of membrane receptors is to negatively regulate signaling by targeting receptors for lysosomal degradation. The high affinity receptor for IgE (FcepsilonRI) expressed on mast cells and basophils is rapidly ubiquitinated upon antigen stimulation. However, the nature and the role of this covalent modification are still largelly unknown. Here, we show that FcepsilonRI subunits are preferentially ubiquitinated at multiple sites upon stimulation, and provide evidence for a role of ubiquitin as an internalization signal: under conditions of impaired receptor ubiquitination a decrease of receptor entry is observed by FACS analysis and fluorescence microscopy. We also used biochemical approaches combined with fluorescence microscopy, to demonstrate that receptor endocytosis requires the integrity of specific membrane domains, namely lipid rafts. Additionally, by RNA interference we demonstrate the involvement of ubiquitin-binding endocytic adaptors in FcepsilonRI internalization and sorting. Notably, the triple depletion of Eps15, Eps15R and Epsin1 negatively affects the early steps of Ag-induced receptor endocytosis, whereas Hrs depletion retains ubiquitinated receptors into early endosomes and partially prevents their sorting into lysosomes for degradation. Our results are compatible with a scenario in which the accumulation of engaged receptor subunits into lipid rafts is required for receptor ubiquitination, a prerequisite for efficient receptor internalization, sorting and delivery to a lysosomal compartment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.