ArticleInternational archives of allergy and immunology2009
NF-kappaB-dependent induction of cathelicidin-related antimicrobial peptide in murine mast cells by lipopolysaccharide.
Article in International archives of allergy and immunology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 55 citations in OpenAlex.
- Molecular Cloning, Expression Analyses, and Physiological Roles of Cathelicidins in the Bursa of Fabricius of the Japanese Quail,Antibiotics (Basel, Switzerland) · 2023Article
- Type-I Interferon Signaling Protects against Chlamydia trachomatis Infection in the Female Lower Genital Tract.Infection and immunity · 2023Article
- Induction of Endogenous Antimicrobial Peptides to Prevent or Treat Oral Infection and Inflammation.Antibiotics (Basel, Switzerland) · 2023Review
- Role of antimicrobial peptide cathelicidin in thrombosis and thromboinflammation.Frontiers in immunology · 2023Review
- 25-Hydroxyvitamin D potentializes extracellular cathelicidin release from human PBMC stimulated ex vivo with either bacterial (LPS) or viral (P: IC) mimetics.Journal of physiology and biochemistry · 2022Article
- Lentinan administration alleviates diarrhea of rotavirus-infected weaned pigs via regulating intestinal immunity.Journal of animal science and biotechnology · 2021Article
- Requirement of CRAMP for mouse macrophages to eliminate phagocytosedJournal of cell science · 2021Article
- Regulation of CAMP (cathelicidin antimicrobial peptide) expression in adipocytes by TLR 2 and 4.Innate immunity · 2021Article
- Review
- Characterization of a fungal competition factor: Production of a conidial cell-wall associated antifungal peptide.PLoS pathogens · 2020Article
- Differential Expression of Antimicrobial Peptides in Streptococcus pneumoniae Keratitis and STAT3-Dependent Expression of LL-37 by Streptococcus pneumoniae in Human Corneal Epithelial Cells.Pathogens (Basel, Switzerland) · 2019Article
- Mast cells participate in regulation of lung-gut axis during Staphylococcus aureus pneumonia.Cell proliferation · 2019Article
- The Inhibitory Innate Immune Sensor NLRP12 Maintains a Threshold against Obesity by Regulating Gut Microbiota Homeostasis.Cell host & microbe · 2018Article
- Toll-like receptors in immunity and inflammatory diseases: Past, present, and future.International immunopharmacology · 2018Review
- Immune Characterization of Bone Marrow-Derived Models of Mucosal and Connective Tissue Mast Cells.Allergy, asthma & immunology research · 2018Article
- Mouse Bone Marrow Sca-1Infection and immunity · 2017Article
- Skin microbiome and mast cells.Translational research : the journal of laboratory and clinical medicine · 2017Review
- The 11S Proteasome Subunit PSME3 Is a Positive Feedforward Regulator of NF-κB and Important for Host Defense against Bacterial Pathogens.Cell reports · 2016Article
- LL-37 peptide enhancement of signal transduction by Toll-like receptor 3 is regulated by pH: identification of a peptide antagonist of LL-37.The Journal of biological chemistry · 2014Article
- Endogenous cathelicidin production limits inflammation and protective immunity to Mycobacterium avium in mice.Immunity, inflammation and disease · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAn important aspect of the innate immune response to pathogens is the production of anti-microbial peptides such as cathelicidin-related antimicrobial peptide (CRAMP), the murine homologue of human cathelicidin LL-37. In this study, mechanisms regulating LPS-induction of CRAMP gene expression in mast cells were investigated. NF-kappaB and MAPK pathways were the focus of investigation.
methodsMouse bone marrow-derived mast cells were grown in culture and stimulated with LPS. MAPKs and NF-kappaB were monitored by immunoblot analysis. ERK, JNK and p38 MAPK were inhibited using siRNAs or a pharmacological inhibitor. Accumulation of the p65 component of NF-kappaB was inhibited by siRNA and NF-kappaB activation was inhibited by overexpression of I kappaB alpha. MEKK2 or MEKK3 were overexpressed by transfection. The effects of all of these treatments on CRAMP gene expression were monitored by RT-PCR.
resultsInhibition of ERK, JNK or p38 MAPK had little discernible effect on LPS-inducible CRAMP gene expression. Overexpression of MEKK2 or MEKK3 likewise had little impact. However, inhibition of the accumulation of p65 NF-kappaB prevented LPS-induced CRAMP mRNA. An important role for NF-kappaB in CRAMP gene expression was confirmed by overexpression of I kappaB alpha, which reduced both basal and induced levels of CRAMP mRNA.
conclusionsNF-kappaB, but not MAPKs, plays an important role in LPS-mediated induction of CRAMP gene in mast cells. Defects which inhibit NF-kappaB activity may increase susceptibility to bacterial and viral pathogens which are sensitive to cathelicidins.
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