ArticleJournal of clinical microbiology2009
Comparison of two multiple-locus variable-number tandem-repeat analysis methods for molecular strain typing of human Brucella melitensis isolates from the Middle East.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- East Mediterranean Lineage ofF1000Research · 2025Article
- Whole-Genome Sequencing for Tracing the Genetic Diversity ofPathogens (Basel, Switzerland) · 2021Article
- MLVA-16 Genotyping ofPathogens (Basel, Switzerland) · 2020Article
- Phenotypic and genotypic characteristics of Brucella isolates from the Republic of Kazakhstan.Tropical animal health and production · 2019Article
- Genome Sequences of Human and Livestock Isolates ofGenome announcements · 2017Article
- African LineageFrontiers in microbiology · 2017Article
- The draft genome ofStandards in genomic sciences · 2017Article
- Human Brucella canis Infection and Subsequent Laboratory Exposures Associated with a Puppy, New York City, 2012.Zoonoses and public health · 2015Article
- Brucella placentitis and seroprevalence in northern fur seals ( Callorhinus ursinus) of the Pribilof Islands, Alaska.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2014Article
- Evaluation and Selection of Multilocus Variable-Number Tandem-Repeat Analysis Primers for Genotyping Brucella abortus Biovar 1 Isolated from Human Patients.Osong public health and research perspectives · 2013Article
- Review of brucellosis cases from laboratory exposures in the United States in 2008 to 2011 and improved strategies for disease prevention.Journal of clinical microbiology · 2013Review
- Molecular epidemiology of Brucella abortus isolates from cattle, elk, and bison in the United States, 1998 to 2011.Applied and environmental microbiology · 2012Article
- Molecular typing of Brucella melitensis endemic strains and differentiation from the vaccine strain Rev-1.Veterinary research communications · 2012Article
- MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates.BMC microbiology · 2011Article
- Characterization of novel Brucella strains originating from wild native rodent species in North Queensland, Australia.Applied and environmental microbiology · 2010Article
- Epidemiological and phylogenetic analysis of Spanish human Brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpoB typing.Journal of clinical microbiology · 2010Article
- Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis.Journal of clinical microbiology · 2010Article
- Identification of an unusual Brucella strain (BO2) from a lung biopsy in a 52 year-old patient with chronic destructive pneumonia.BMC microbiology · 2010Article
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13 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Brucella species are highly monomorphic, with minimal genetic variation among species, hindering the development of reliable subtyping tools for epidemiologic and phylogenetic analyses. Our objective was to compare two distinct multiple-locus variable-number tandem-repeat analysis (MLVA) subtyping methods on a collection of 101 Brucella melitensis isolates from sporadic human cases of brucellosis in Egypt (n = 83), Qatar (n = 17), and Libya (n = 1). A gel-based MLVA technique, MLVA-15(IGM), was compared to an automated capillary electrophoresis-based method, MLVA-15(NAU), with each MLVA scheme examining a unique set of variable-number tandem repeats. Both the MLVA(IGM) and MLVA(NAU) methods were highly discriminatory, resolving 99 and 101 distinct genotypes, respectively, and were able to largely separate genotypes from Egypt and Qatar. The MLVA-15(NAU) scheme presented higher strain-to-strain diversity in our test population than that observed with the MLVA-15(IGM) assay. Both schemes were able to genetically correlate some strains originating from the same hospital or region within a country. In addition to comparing the genotyping abilities of these two schemes, we also compared the usability, limitations, and advantages of the two MLVA systems and their applications in the epidemiological genotyping of human B. melitensis strains.
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