ArticleJournal of clinical microbiology2009
Real-time PCR-based pathotyping of Newcastle disease virus by use of TaqMan minor groove binder probes.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 50 citations in OpenAlex.
- High-sensitivity multiplex real-time RT-PCR for universal detection and virulence typing of Newcastle disease virus in poultry field samples.Poultry science · 2026Article
- Optimization and Validation of Universal Real-Time RT-PCR Assay to Detect Virulent Newcastle Disease Viruses.Viruses · 2025Article
- Detection of Newcastle disease virus and assessment of associated relative risk in backyard and commercial poultry in Kerala, India.Veterinary medicine and science · 2022Article
- Prevalence of Newcastle disease and associated risk factors in domestic chickens in the Indian state of Odisha.PloS one · 2022Article
- Predominance of Fourth Panzootic Newcastle Disease Virus Subgenotype VII.1.1 in Iran and Its Relation to the Genotypes Circulating in the Region.Current microbiology · 2021Article
- Prevalence of Newcastle Disease Virus in Commercial and Backyard Poultry in Haryana, India.Frontiers in veterinary science · 2021Article
- Generation and evaluation of a vaccine candidate of attenuated and heat-resistant genotype VIII Newcastle disease virus.Poultry science · 2020Article
- Simultaneous and visual detection of infectious bronchitis virus and Newcastle disease virus by multiple LAMP and lateral flow dipstick.Poultry science · 2019Article
- Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases.BMC veterinary research · 2019Article
- Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives.BioMed research international · 2018Review
- Accurate Detection of Avian Respiratory Viruses by Use of Multiplex PCR-Based Luminex Suspension Microarray Assay.Journal of clinical microbiology · 2016Article
- Detection of Newcastle disease virus minor genetic variants by modified single-stranded conformational polymorphism analysis.BioMed research international · 2014Article
- Avian paramyxovirus serotype-1: a review of disease distribution, clinical symptoms, and laboratory diagnostics.Veterinary medicine international · 2012Article
- Detection and differentiation of virulent and avirulent strains of Newcastle disease virus by real-time PCR.Journal of virological methods · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time reverse-transcription PCR was developed to detect and pathotype Newcastle disease viruses (NDV) in clinical samples. Degenerate oligonucleotide primers and TaqMan probes with nonfluorescent minor groove binder (MGB) quencher amplified and hybridized to a region in the fusion protein (F) gene that corresponds to the cleavage site of the F0 precursor, which is a key determinant of NDV pathogenicity. The application of degenerate primers and TaqMan MGB probes provided high specificity to the assay, as was shown by the successful and rapid pathotype determination of 39 NDV strains representing all the known genotypes (I to VIII) and pathotypes (lentogens/mesogens/velogens). The PCR assays specific for lentogenic and velogenic/mesogenic strains had high analytical sensitivity, detecting approximately 10 and 20 copies of the target molecule per reaction, respectively. The detection limit was also determined in terms of 50% egg infective dose (EID(50)) by using dilution series of virus stock solutions to be approximately 10(1.0) and 10(-1.3) EID(50)/ml for lentogens and velogens/mesogens, respectively. Organ, swab, and stool specimens from experimentally infected animals were tested to prove the clinical suitability of the method. The results of this study suggest that the described real-time PCR assay has the potential to be used for the rapid detection/pathotyping of NDV isolates and qualitative/quantitative measurement of the virus load.
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