ArticleClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases2010
Dual detection of Legionella pneumophila and Legionella species by real-time PCR targeting the 23S-5S rRNA gene spacer region.
Article in Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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22 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Diagnostic Accuracy of PCR Alone and Compared to Urinary Antigen Testing for Detection of Legionella spp.: a Systematic Review.Journal of clinical microbiology · 2016Pooled it
- Impact of COVID-19 pandemic on Legionella testing and infection rates in Ontario.BMC public health · 2025Article
- Development of a PCR Assay for the Detection ofInfectious disease reports · 2025Article
- Two-stage surveillance ofSAGE open medicine · 2025Article
- Design of an optical nanobiosensor for detection ofIranian journal of microbiology · 2022Article
- Comparison of the culture method with multiplex PCR for the confirmation of Legionella spp. and Legionella pneumophila.Journal of applied microbiology · 2021Article
- Cooccurrence of Five PathogenicPathogens (Basel, Switzerland) · 2021Article
- Enhancement of Culture of Legionella longbeachae from Respiratory Samples by Use of Immunomagnetic Separation and Antimicrobial Decontamination.Journal of clinical microbiology · 2020Article
- Isolation and Identification ofAIMS microbiology · 2019Article
- Loop-mediated isothermal amplification for detection ofInfection and drug resistance · 2019Article
- Legionella pneumonia in the Niagara Region, Ontario, Canada: a case series.Journal of medical case reports · 2016Observational
- PCR method for the rapid detection and discrimination of Legionella spp. based on the amplification of pcs, pmtA, and 16S rRNA genes.Journal of applied genetics · 2016Article
- Evaluation and Comparison of Multiple Test Methods, Including Real-time PCR, for Legionella Detection in Clinical Specimens.Frontiers in public health · 2016Article
- Acute Respiratory Infections in Travelers Returning from MERS-CoV-Affected Areas.Emerging infectious diseases · 2015Article
- A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp.PloS one · 2014Article
- Clinical application of a multiplex real-time PCR assay for simultaneous detection of Legionella species, Legionella pneumophila, and Legionella pneumophila serogroup 1.Journal of clinical microbiology · 2013Article
- Detection of Legionella pneumophila on clinical samples and susceptibility assessment by flow cytometry.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Application of Legionella pneumophila-specific quantitative real-time PCR combined with direct amplification and sequence-based typing in the diagnosis and epidemiological investigation of Legionnaires' disease.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Application of TaqMan low-density arrays for simultaneous detection of multiple respiratory pathogens.Journal of clinical microbiology · 2011Article
- Rapid qualitative urinary tract infection pathogen identification by SeptiFast real-time PCR.PloS one · 2011Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the majority of cases of Legionnaires' disease (LD) are caused by Legionella pneumophila, an increasing number of other Legionella species have been reported to cause human disease. There are no clinical presentations unique to LD and hence accurate laboratory tests are required for early diagnosis. Therefore, we designed a real-time PCR assay that targets the 23S-5S rRNA intergenic spacer region (23S-5S PCR) and allows for detection of all Legionella species and discrimination of L. pneumophila from other Legionella species. In total, 271 isolates representing 50 Legionella species were tested and the assay was validated using 39 culture-positive and 110 culture-negative patient specimens collected between 1989 and 2006. PCR-positive results were obtained with all 39 culture-positive samples (100% sensitivity). Specimens that tested positive according to 23S-5S PCR, but were culture-negative, were further analysed by DNA sequencing of the amplicon or the macrophage infectivity potentiator (mip) gene. In addition to L. pneumophila, Legionella longbeachae, Legionella cincinnatiensis and Legionella micdadei were identified in the specimens. The assay showed a 7-log dynamic range displaying a sensitivity of 7.5 CFU/mL or three genome equivalents per reaction. Sixty-one specimens containing viruses or bacteria other than Legionellae were negative according to 23S-5S PCR, demonstrating its specificity. Use of this assay should contribute to the earlier detection of respiratory disease caused by Legionella species, as well as to increased rates of detection.
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