ArticleHFSP journal2008
Ruggedness in the folding landscape of protein L.
Article in HFSP journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Protein Structural Characterization Using Electron Transfer Dissociation and Hydrogen Exchange-Mass Spectrometry.Bio-protocol · 2025Article
- How cooperative are protein folding and unfolding transitions?Protein science : a publication of the Protein Society · 2016Review
- Complex pathways in folding of protein G explored by simulation and experiment.Biophysical journal · 2014Article
- Fast protein folding kinetics.Quarterly reviews of biophysics · 2014Review
- Small-angle X-ray scattering and single-molecule FRET spectroscopy produce highly divergent views of the low-denaturant unfolded state.Journal of molecular biology · 2012Article
- Microfluidic mixers for studying protein folding.Journal of visualized experiments : JoVE · 2012Article
- How, when and why proteins collapse: the relation to folding.Current opinion in structural biology · 2012Review
- Taming the complexity of protein folding.Current opinion in structural biology · 2011Review
- Extremely slow intramolecular diffusion in unfolded protein L.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Protein folded states are kinetic hubs.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Unfolded-state dynamics and structure of protein L characterized by simulation and experiment.Journal of the American Chemical Society · 2010Article
- Direct observation of downhill folding of lambda-repressor in a microfluidic mixer.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By exploring the folding pathways of the B1 domain of protein L with a series of equilibrium and rapid kinetic experiments, we have found its unfolded state to be more complex than suggested by two-state folding models. Using an ultrarapid mixer to initiate protein folding within approximately 2-4 microseconds, we observe folding kinetics by intrinsic tryptophan fluorescence and fluorescence resonance energy transfer. We detect at least two processes faster than 100 mus that would be hidden within the burst phase of a stopped-flow instrument measuring tryptophan fluorescence. Previously reported measurements of slow intramolecular diffusion are commensurate with the slower of the two observed fast phases. These results suggest that a multidimensional energy landscape is necessary to describe the folding of protein L, and that the dynamics of the unfolded state is dominated by multiple small energy barriers.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.