ArticleMolecular therapy : the journal of the American Society of Gene Therapy2009
Heat shock protein 27 as a new therapeutic target for radiation sensitization of head and neck squamous cell carcinoma.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 56 citations in OpenAlex.
- Heat Shock Protein 27 in Radiation-Induced Trismus: Mechanistic Insights and a Hypothesis-Generating Framework.Biomedicines · 2026Review
- HSP90B1 facilitates glioma radiotherapy resistance by regulating RhoC ubiquitin‒proteasome degradation.Genes & diseases · 2026Article
- Review
- Review
- The Interplay between Heat Shock Proteins and Cancer Pathogenesis: A Novel Strategy for Cancer Therapeutics.Cancers · 2024Review
- Profiling of gene expression in the brain associated with anxiety-related behaviors in the chronic phase following cranial irradiation.Scientific reports · 2022Article
- Review
- Hypoxia-Induced Cancer Cell Responses Driving Radioresistance of Hypoxic Tumors: Approaches to Targeting and Radiosensitizing.Cancers · 2021Review
- Involvement of CDKN1A (p21) in cellular senescence in response to heat and irradiation stress during preimplantation development.Cell stress & chaperones · 2020Article
- KNK437 restricts the growth and metastasis of colorectal cancer via targeting DNAJA1/CDC45 axis.Oncogene · 2020Article
- Review
- Hsp27 regulates epithelial mesenchymal transition, metastasis and proliferation in colorectal carcinoma.Oncology letters · 2018Article
- Clinical, prognostic, and therapeutic significance of heat shock protein 27 in bladder cancer.Oncotarget · 2018Article
- The clinicopathological and prognostic value of HSP27 in hepatocellular carcinoma: a systematic review and meta-analysis.OncoTargets and therapy · 2018Article
- Role of Krüppel-like factor 4 and heat shock protein 27 in cancer of the larynx.Molecular and clinical oncology · 2017Article
- Article
- Article
- Overcoming HSP27-mediated resistance by altered dimerization of HSP27 using small molecules.Oncotarget · 2016Article
- A Targetable Molecular Chaperone Hsp27 Confers Aggressiveness in Hepatocellular Carcinoma.Theranostics · 2016Article
- HSP27 Knockdown Increases Cytoplasmic p21 and Cisplatin Sensitivity in Ovarian Carcinoma Cells.Oncology research · 2016Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a wide range of human cancers, increased levels of heat shock protein 27 (Hsp27) are closely associated with tumorigenesis, metastasis, resistance to anticancer therapeutics, and thus poor prognosis. In this study, we evaluate the radiosensitizing effects of Hsp27 gene silencing using OGX-427, a second-generation antisense oligonucleotide (ASO), on the radioresistant head and neck squamous cell carcinoma (HNSCC) SQ20B cells. In vitro, the downregulation of Hsp27 significantly enhanced radiation-induced apoptotic and clonogenic death, and promoted Akt inactivation. In vivo, combining OGX-427 with local tumor irradiation (5 x 2 Gy) led to a significant regression of SQ20B tumors related to a high rate of apoptosis and decreased levels of glutathione antioxidant defenses. Increasing the total radiation dose (15 x 2 Gy) significantly amplified the radiosensitizing effect of OGX-427. Treatment of tumors with OGX-427 plus radiation resulted in a decrease in angiogenesis associated with a reduced activation of the Akt pathway. Furthermore, the combined treatment enhanced the survival of SQ20B-bearing mice and showed no signs of acute and delayed toxicity. Our findings demonstrate for the first time that Hsp27 knockdown enhances the cytotoxic effects of radiotherapy in vivo and provide preclinical proof of principle for clinical trials using Hsp27 antisense technology in the treatment of patients with HNSCC radioresistant cancers.
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