ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2009
Localization of mRNAs and proteins in methyl methacrylate-embedded tissues.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 14 citations in OpenAlex.
- Ultraplex microscopy: versatile highly-multiplexed molecular labeling and imaging across scale and resolution.bioRxiv : the preprint server for biology · 2024Article
- Streamlined Full-Length Total RNA Sequencing of Paraformaldehyde-Fixed Brain Tissues.International journal of molecular sciences · 2024Article
- Form and function of the intervertebral disc in health and disease: a morphological and stain comparison study.Journal of anatomy · 2015Article
- Morphological and molecular characterization of developing vertebral fusions using a teleost model.BMC physiology · 2010Article
- Molecular pathology of vertebral deformities in hyperthermic Atlantic salmon (Salmo salar).BMC physiology · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
Precise localization of proteins and mRNA in histological sections is necessary for evaluating spatial gene expression patterns. Here we report sensitive detection of the gene products in fish tissues by immunohistochemistry (IHC) and in situ hybridization (ISH) assays on sections of whole specimens and vertebra embedded in methyl methacrylate (MMA) resin. This plastic resin favors easy preparation of various specimen types and enables preparation of large sections with well-preserved cell morphology. IHC analysis of the muscle regulatory factor MyoD in transverse sections of juvenile cod revealed MyoD-positive cells in the dorsolateral parts of the adaxial muscle. ISH revealed less spatially restricted signals of the bone morphogenic protein bmp4 in muscle and brain. To assess the applicability of ISH on sections of bony tissue, col1a1 and col2a1 expression was investigated in non-decalcified vertebra sections of Atlantic salmon. The former was identified in both chondrocytes and osteoblasts, whereas the latter was mostly evident in chondrocytes. We conclude that MMA resin offers easy preparation of large and problematic tissues and the possibility of carrying out both IHC and ISH analyses using standard protocols.
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