ArticleBioorganic & medicinal chemistry letters2009
CSI-FID: high throughput label-free detection of DNA binding molecules.
Article in Bioorganic & medicinal chemistry letters, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Using synthetic genome readers/regulators to interrogate chromatin processes: A brief review.Methods (San Diego, Calif.) · 2024Review
- A novel molecular rotor facilitates detection of p53-DNA interactions using the Fluorescent Intercalator Displacement Assay.Scientific reports · 2018Article
- Article
- Synthetic genome readers target clustered binding sites across diverse chromatin states.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin).Journal of visualized experiments : JoVE · 2016Article
- Dry and wet approaches for genome-wide functional annotation of conventional and unconventional transcriptional activators.Computational and structural biotechnology journal · 2016Review
- Mapping polyamide-DNA interactions in human cells reveals a new design strategy for effective targeting of genomic sites.Angewandte Chemie (International ed. in English) · 2014Article
- Discovery libraries targeting the major enzyme classes: the serine hydrolases.Bioorganic & medicinal chemistry letters · 2014Article
- Melting thermodynamics of reversible DNA/ligand complexes at interfaces.Journal of the American Chemical Society · 2012Article
- Fluorescent intercalator displacement replacement (FIDR) assay: determination of relative thermodynamic and kinetic parameters in triplex formation--a case study using triplex-forming LNAs.Nucleic acids research · 2012Article
- Discovery of HIV fusion inhibitors targeting gp41 using a comprehensive α-helix mimetic library.Bioorganic & medicinal chemistry letters · 2012Article
- Design, synthesis, and validation of a β-turn mimetic library targeting protein-protein and peptide-receptor interactions.Journal of the American Chemical Society · 2011Article
- A modified bacterial one-hybrid system yields improved quantitative models of transcription factor specificity.Nucleic acids research · 2011Article
- Determining the specificity of protein-DNA interactions.Nature reviews. Genetics · 2010Review
- Semi-automated high-throughput fluorescent intercalator displacement-based discovery of cytotoxic DNA binding agents from a large compound library.Bioorganic & medicinal chemistry letters · 2010Article
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Authors and funding
4 authors.
Funding
Abstract
Determining the sequence specifity of DNA binding molecules is a non-trivial task. Here we describe the development of a platform for assaying the sequence specificity of DNA ligands using label free detection on high density DNA microarrays. This is achieved by combining Cognate Site Identification (CSI) with Fluorescence Intercalation Displacement (FID) to create CSI-FID. We use the well-studied small molecule DNA ligand netropsin to develop this high throughput platform. Analysis of the DNA binding properties of protein- and small molecule-based libraries with CSI-FID will advance the development of genome-anchored molecules for therapeutic purposes.
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