ArticleAnalytical chemistry2009
Optimization of encoded hydrogel particles for nucleic acid quantification.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Novabeads: Stimuli-Responsive Signal-Amplifying Hydrogel Microparticles for Enzymeless Fluorescence-Based Detection of microRNA Biomarkers.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Polyethylene Glycol Nanofiller for Robust Lyophilization of Graphically Encoded Hydrogel Microparticles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Multiplex Assay for Rapid Detection and Analysis of Nucleic Acid Using Barcode Receptor Encoded Particle (BREP).Biomedicines · 2022Article
- Quantitative and Multiplex Detection of Extracellular Vesicle-Derived MicroRNA via Rolling Circle Amplification within Encoded Hydrogel Microparticles.Advanced healthcare materials · 2022Article
- High-throughput selection of cells based on accumulated growth and division using PicoShell particles.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Highly Magnetized Encoded Hydrogel Microparticles with Enhanced Rinsing Capabilities for Efficient microRNA Detection.Biomedicines · 2021Article
- In-gel fluorescence detection by DNA polymerase elongation.APL bioengineering · 2020Article
- A platform for multiplexed colorimetric microRNA detection using shape-encoded hydrogel particles.The Analyst · 2020Article
- Hydrogel-Based Colorimetric Assay for Multiplexed MicroRNA Detection in a Microfluidic Device.Analytical chemistry · 2020Article
- Article
- MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically encoded hydrogel beads using a simple single-layer microfluidic device.Microsystems & nanoengineering · 2020Article
- Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays.Microsystems & nanoengineering · 2020Article
- Implantable Photothermal Agents based on Gold Nanorods-Encapsulated Microcube.Scientific reports · 2018Article
- Nonfouling, Encoded Hydrogel Microparticles for Multiplex MicroRNA Profiling Directly from Formalin-Fixed, Paraffin-Embedded Tissue.Analytical chemistry · 2018Article
- Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays.Lab on a chip · 2018Article
- Microparticle parking and isolation for highly sensitive microRNA detection.Lab on a chip · 2017Article
- Bioengineering Microgels and Hydrogel Microparticles for Sensing Biomolecular Targets.Gels (Basel, Switzerland) · 2017Review
- Porous microwells for geometry-selective, large-scale microparticle arrays.Nature materials · 2017Article
- Hydrogel microparticles for biosensing.European polymer journal · 2015Article
- Sensitive and multiplexed on-chip microRNA profiling in oil-isolated hydrogel chambers.Angewandte Chemie (International ed. in English) · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The accurate quantification of nucleic acids is of utmost importance for clinical diagnostics, drug discovery, and basic science research. These applications require the concurrent measurement of multiple targets while demanding high-throughput analysis, high sensitivity, specificity between closely related targets, and a wide dynamic range. In attempt to create a technology that can simultaneously meet these demands, we recently developed a method of multiplexed analysis using encoded hydrogel particles. Here, we demonstrate tuning of hydrogel porosity with semi-interpenetrating networks of poly(ethylene glycol), develop a quantitative model to understand hybridization kinetics, and use the findings from these studies to enhance particle design for nucleic acid detection. With an optimized particle design and efficient fluorescent labeling scheme, we demonstrate subattomole sensitivity and single-nucleotide specificity for small RNA targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.