ArticlePathology oncology research : POR2009
Post-translational regulation of cathepsin B, but not of other cysteine cathepsins, contributes to increased glioblastoma cell invasiveness in vitro.
Article in Pathology oncology research : POR, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Systematic review of protein biomarkers of invasive behavior in glioblastoma.Molecular neurobiology · 2014Pooled it
- Immunostaining protocol for infiltrating brain cancer spheroids for light-sheet imaging.PloS one · 2023Article
- Embedded Human Periodontal Ligament Stem Cells Spheroids Enhance Cementogenic Differentiation via Plasminogen Activator Inhibitor 1.International journal of molecular sciences · 2022Article
- Single-cell tracking reveals super-spreading brain cancer cells with high persistence.Biochemistry and biophysics reports · 2021Article
- Serine protease modulation of Dependence Receptors and EMT protein expression.Cancer biology & therapy · 2019Article
- Cysteine cathepsins B, X and K expression in peri-arteriolar glioblastoma stem cell niches.Journal of molecular histology · 2018Article
- Embedded Spheroids as Models of the Cancer Microenvironment.Advanced biosystems · 2017Article
- Mesenchymal stem cells differentially affect the invasion of distinct glioblastoma cell lines.Oncotarget · 2017Article
- The Mechanics of Single Cell and Collective Migration of Tumor Cells.Journal of biomechanical engineering · 2017Review
- Nitroxoline impairs tumor progression in vitro and in vivo by regulating cathepsin B activity.Oncotarget · 2015Article
- Decreased expression of APAF-1 and increased expression of cathepsin B in invasive pituitary adenoma.OncoTargets and therapy · 2015Article
- Cysteine cathepsins: their role in tumor progression and recent trends in the development of imaging probes.Frontiers in chemistry · 2015Review
- Spherical cancer models in tumor biology.Neoplasia (New York, N.Y.) · 2015Review
- Expression analysis of all protease genes reveals cathepsin K to be overexpressed in glioblastoma.PloS one · 2014Article
- Cerebrospinal fluid cathepsin B and S.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2013Article
- Review
- AGR2 is a novel surface antigen that promotes the dissemination of pancreatic cancer cells through regulation of cathepsins B and D.Cancer research · 2011Article
- CD133/prominin1 is prognostic for GBM patient's survival, but inversely correlated with cysteine cathepsins' expression in glioblastoma derived spheroids.Radiology and oncology · 2011Article
- Cathepsin B is the driving force of esophageal cell invasion in a fibroblast-dependent manner.Neoplasia (New York, N.Y.) · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells that migrate away from a central tumour into brain tissue are responsible for inefficient glioblastoma treatment. This migratory behaviour depends partially on lysosomal cysteine cathepsins. Reportedly, the expression of cathepsins B, L and S gradually increases in the progression from benign astrocytoma to the malignant glioblastoma, although their specific roles in glioma progression have not been revealed. The aim of this study was to clarify their specific contribution to glioblastoma cell invasion. The differences between the matrix invading cells and non-invading core cells from spheroids derived from glioblastoma cell culture and from glioblastoma patients' biopsies, and embedded in type I collagen, have been studied at the mRNA, protein and cathepsin activity levels. Analyses of the two types of cells showed that the three cathepsins were up-regulated post-translationally, their specific activities increasing in the invading cells. The cystatin levels were also differentially altered, resulting in higher ratio of cathepsins B and L to stefin B in the invading cells. However, using specific synthetic inhibitors and silencing strategies revealed that only cathepsin B activity was involved in the invasion of glioblastoma cells, confirming previous notion of cathepsin B as tumour invasiveness biomarker. Our data support the concept of specific roles of cysteine cathepsins in cancer progression. Finally the study points out on the complexity of protease regulation and the need to include functional proteomics in the systems biology approaches to understand the processes associated with glioma invasion and progression.
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