ArticleTransgenic research2009
Seed-specific expression of truncated OsGAD2 produces GABA-enriched rice grains that influence a decrease in blood pressure in spontaneously hypertensive rats.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Double-truncated version of OsGADs leads to higher GABA accumulation and stronger stress tolerance in Oryza sativa L. var. japonica.Plant cell reports · 2025Article
- Contributions of Gamma-Aminobutyric Acid (GABA) Produced by Lactic Acid Bacteria on Food Quality and Human Health: Current Applications and Future Prospects.Foods (Basel, Switzerland) · 2024Review
- Truncation of the calmodulin binding domain in rice glutamate decarboxylase 4 (Molecular breeding : new strategies in plant improvement · 2024Article
- From qualitative to quantitative: the state of the art and challenges for plant synthetic biology.Quantitative biology (Beijing, China) · 2023Review
- Genes and Their Molecular Functions Determining Seed Structure, Components, and Quality of Rice.Rice (New York, N.Y.) · 2022Review
- Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle.NPJ science of food · 2021Article
- Rice Compounds with Impact on Diabetes Control.Foods (Basel, Switzerland) · 2021Review
- Spider Silk Fibroin Protein Heterologously Produced in Rice Seeds Reduce Diabetes and Hypercholesterolemia in Mice.Plants (Basel, Switzerland) · 2020Article
- Improving nutritional quality of rice for human health.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- An In Vivo Targeted Deletion of the Calmodulin-Binding Domain from Rice Glutamate Decarboxylase 3 (OsGAD3) Increases γ-Aminobutyric Acid Content in Grains.Rice (New York, N.Y.) · 2020Article
- An Updated Review on Pharmaceutical Properties of Gamma-Aminobutyric Acid.Molecules (Basel, Switzerland) · 2019Review
- Field trial of GABA-fortified rice plants and oral administration of milled rice in spontaneously hypertensive rats.Transgenic research · 2015Article
- Treatment of GABA from Fermented Rice Germ Ameliorates Caffeine-Induced Sleep Disturbance in Mice.Biomolecules & therapeutics · 2015Article
- Identification and transcript analysis of two glutamate decarboxylase genes, CsGAD1 and CsGAD2, reveal the strong relationship between CsGAD1 and citrate utilization in citrus fruit.Molecular biology reports · 2014Article
- Determination of GABA(Aα1) and GABA (B1) receptor subunits expression in tissues of gilts during the late gestation.Molecular biology reports · 2013Article
- Changes in primary and secondary metabolite levels in response to gene targeting-mediated site-directed mutagenesis of the anthranilate synthase gene in rice.Metabolites · 2012Article
- Beta-adrenergic signaling in the development and progression of pulmonary and pancreatic adenocarcinoma.Current cancer therapy reviews · 2012Article
- Targeted enhancement of glutamate-to-γ-aminobutyrate conversion in Arabidopsis seeds affects carbon-nitrogen balance and storage reserves in a development-dependent manner.Plant physiology · 2011Article
- Functional genomics of rice pollen and seed development by genome-wide transcript profiling and Ds insertion mutagenesis.International journal of biological sciences · 2010Article
- The potential bioproduction of the pharmaceutical agent sakuranetin, a flavonoid phytoalexin in rice.BioengineeredArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gamma-aminobutyric acid (GABA) is a four-carbon amino acid that is commonly present in living organisms and functions as a major inhibitory neurotransmitter in mammals. It is understood to have a potentially anti-hypertensive effect in mammals. GABA is synthesized from glutamate by glutamate decarboxylase (GAD). In plants, GAD is regulated via its calmodulin-binding domain (CaMBD) by Ca(2+)/CaM. We have previously reported that a C-terminal truncated version of one of the five rice GAD isoforms, GAD2DeltaC, revealed higher enzymatic activity in vitro and that its over-expression resulted in exceptionally high GABA accumulation (Akama and Takaiwa, J Exp Bot 58:2699-2607, 2007). In this study, GAD2DeltaC, under the control of the rice glutelin promoter (GluB-1), was introduced into rice cells via Agrobacterium-mediated transformation to produce transgenic rice lines. Analysis of the free amino acid content of rice grains revealed up to about a 30-fold higher level of GABA than in non-transformed rice grains. There were also very high levels of various free protein amino acids in the seeds. GABA-enriched rice grains were milled to a fine powder for oral administration to spontaneously hypertensive rats (SHRs) and normotensive Wistar-Kyoto rats (WKYs). Six weeks of administration showed that transgenic rice brings about a 20 mmHg decrease in blood pressure in two different kinds of SHRs, while there was no significant hypotensive effect in WKYs. These results suggest an alternative way to control and/or cure hypertension in humans with GABA-enriched rice as part of a common daily diet.
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