ArticleGlycobiology2009
Targeted glycoproteomic identification of cancer cell glycosylation.
Article in Glycobiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 69 citations in OpenAlex.
- N-Glycosylation of AXL Receptor Tyrosine Kinase Regulates Its Stability, Phosphorylation, and Oncogenic Function.Molecular & cellular proteomics : MCP · 2026Article
- Use of a glycomics array to establish the anti-carbohydrate antibody repertoire in type 1 diabetes.Nature communications · 2022Article
- Multiplex Glycan Bead Array (MGBA ) for High Throughput and High Content Analyses of Glycan-Binding Proteins Including Natural Anti-Glycan Antibodies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Antiproliferative and Antimicrobial Potentials of a Lectin fromMarine drugs · 2021Article
- Detection of Aberrant Glycosylation of Serum Haptoglobin for Gastric Cancer Diagnosis Using a Middle-Up-Down Glycoproteome Platform.Journal of personalized medicine · 2021Article
- Comparison of the sensitivity of Western blotting between PVDF and NC membranes.Scientific reports · 2021Article
- Internalization of Garlic-Derived Nanovesicles on Liver Cells is Triggered by Interaction With CD98.ACS omega · 2020Article
- Enhanced Immune Response Against the Thomsen-Friedenreich Tumor Antigen Using a Bivalent Entirely Carbohydrate Conjugate.Molecules (Basel, Switzerland) · 2020Article
- Identification of serum glycoprotein ligands for the immunomodulatory receptor blood dendritic cell antigen 2.Glycobiology · 2018Article
- Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins.Nature communications · 2018Article
- Select membrane proteins modulate MNV-1 infection of macrophages and dendritic cells in a cell type-specific manner.Virus research · 2016Article
- Glycomics and glycoproteomics of membrane proteins and cell-surface receptors: Present trends and future opportunities.Electrophoresis · 2016Review
- Insights into glycan biosynthesis in chemically-induced hepatocellular carcinoma in rats: A glycomic analysis.World journal of gastroenterology · 2015Article
- Marine lectins and their medicinal applications.Applied microbiology and biotechnology · 2015Review
- Cell surface nucleolin facilitates enterovirus 71 binding and infection.Journal of virology · 2015Article
- Glycomic profiling of carcinoembryonic antigen isolated from human tumor tissue.Clinical proteomics · 2015Article
- Snake venom galactoside-binding lectins: a structural and functional overview.The journal of venomous animals and toxins including tropical diseases · 2015Article
- Recognition of the Thomsen-Friedenreich pancarcinoma carbohydrate antigen by a lamprey variable lymphocyte receptor.The Journal of biological chemistry · 2013Article
- Implications on glycobiological aspects of tumor hypoxia in breast ductal carcinoma in situ.Medical molecular morphology · 2013Article
- Sugar-binding proteins from fish: selection of high affinity "lambodies" that recognize biomedically relevant glycans.ACS chemical biology · 2013Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
GalMBP is a fragment of serum mannose-binding protein that has been modified to create a probe for galactose-containing ligands. Glycan array screening demonstrated that the carbohydrate-recognition domain of GalMBP selectively binds common groups of tumor-associated glycans, including Lewis-type structures and T antigen, suggesting that engineered glycan-binding proteins such as GalMBP represent novel tools for the characterization of glycoproteins bearing tumor-associated glycans. Blotting of cell extracts and membranes from MCF7 breast cancer cells with radiolabeled GalMBP was used to demonstrate that it binds to a selected set of high molecular weight glycoproteins that could be purified from MCF7 cells on an affinity column constructed with GalMBP. Proteomic and glycomic analysis of these glycoproteins by mass spectrometry showed that they are forms of CD98hc that bear glycans displaying heavily fucosylated termini, including Lewis(x) and Lewis(y) structures. The pool of ligands was found to include the target ligands for anti-CD15 antibodies, which are commonly used to detect Lewis(x) antigen on tumors, and for the endothelial scavenger receptor C-type lectin, which may be involved in tumor metastasis through interactions with this antigen. A survey of additional breast cancer cell lines reveals that there is wide variation in the types of glycosylation that lead to binding of GalMBP. Higher levels of binding are associated either with the presence of outer-arm fucosylated structures carried on a variety of different cell surface glycoproteins or with the presence of high levels of the mucin MUC1 bearing T antigen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.