ArticleInfection and immunity2009
Genetic requirements for Klebsiella pneumoniae-induced liver abscess in an oral infection model.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 89 citations in OpenAlex.
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- Gut Commensal Klebsiella pneumoniae Extracellular Vesicles Shape a Liver Microenvironment Conducive to Gut-Liver Bacterial Translocation and Pro-Tumorigenic Processes.Journal of extracellular vesicles · 2026Article
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- A Case Series of HypermucoidClinical case reports · 2026Article
- Targeting the capsule ofFrontiers in immunology · 2026Article
- Divergent roles for complement components C3 and C4 in controllingbioRxiv : the preprint server for biology · 2025Article
- Genomic Characterization of a Rare K30-ST198 HypervirulentInternational journal of molecular sciences · 2025Article
- Multidrug-resistant hypervirulentFuture microbiology · 2025Review
- Mobile genetic elements in shapingFrontiers in microbiology · 2025Review
- Development and validation of a clinical-radiomics nomogram for the early prediction of Klebsiella pneumoniae liver abscess.Annals of medicine · 2024Article
- Liver abscess induced by intestinal hypervirulentiScience · 2024Article
- An Enteric Bacterial Infection Triggers Neuroinflammation and Neurobehavioral Impairment in 3xTg-AD Transgenic Mice.The Journal of infectious diseases · 2024Article
- Epidemiological and Molecular Characteristics of Hypermucoviscous and HypervirulentInfection and drug resistance · 2024Article
- Alterations of Gut Microbiota in Pyogenic Liver Abscess Patients with and without Type 2 Diabetes Mellitus.Infection and drug resistance · 2024Article
- Genetic and immune determinants ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Pyogenic liver abscess complicated with endogenous endophthalmitis caused by Klebsiella pneumoniae: A case report and Literature Review.Immunity, inflammation and disease · 2023Review
6 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Klebsiella pneumoniae is the predominant pathogen of primary liver abscess. However, our knowledge regarding the molecular basis of how K. pneumoniae causes primary infection in the liver is limited. We established an oral infection model that recapitulated the characteristics of liver abscess and conducted a genetic screen to identify the K. pneumoniae genes required for the development of liver abscess in mice. Twenty-eight mutants with attenuated growth in liver or spleen samples out of 2,880 signature-tagged mutants that produced the wild-type capsule were identified, and genetic loci which were disrupted in these mutants were identified to encode products with roles in cellular metabolism, adhesion, transportation, gene regulation, and unknown functions. We further evaluated the virulence attenuation of these mutants in independent infection experiments and categorized them accordingly into three classes. In particular, the class I and II mutant strains exhibited significantly reduced virulence in mice, and most of these strains were not detected in extraintestinal tissues at 48 h after oral inoculation. Interestingly, the mutated loci of about one-third of the class I and II mutant strains encode proteins with regulatory functions, and the transcript abundances of many other genes identified in the same screen were markedly changed in these regulatory mutant strains, suggesting a requirement for genetic regulatory networks for translocation of K. pneumoniae across the intestinal barrier. Furthermore, our finding that preimmunization with certain class I mutant strains protected mice against challenge with the wild-type strain implied a potential application for these strains in prophylaxis against K. pneumoniae infections.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.