ArticleBMC microbiology2009
Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Frequency Variation and Dose Modification of Benznidazole Administration for the Treatment of Trypanosoma cruzi Infection in Mice, Dogs, and Nonhuman Primates.Antimicrobial agents and chemotherapy · 2023Article
- Frequency variation and dose modification of benznidazole administration for the treatment ofbioRxiv : the preprint server for biology · 2023Article
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- Strain-specific genome evolution in Trypanosoma cruzi, the agent of Chagas disease.PLoS pathogens · 2021Article
- A modified drug regimen clears active and dormant trypanosomes in mouse models of Chagas disease.Science translational medicine · 2020Article
- CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms.Nature reviews. Microbiology · 2018Review
- Expanding an expanded genome: long-read sequencing of Trypanosoma cruzi.Microbial genomics · 2018Article
- Article
- Article
- Article
- Construction of three new Gateway® expression plasmids for Trypanosoma cruzi.Memorias do Instituto Oswaldo Cruz · 2014Article
- Knockout confirmation for Hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture.Memorias do Instituto Oswaldo Cruz · 2014Article
- Biochemical and genetic characterization of Trypanosoma cruzi N-myristoyltransferase.The Biochemical journal · 2014Article
- Plasmid vectors and molecular building blocks for the development of genetic manipulation tools for Trypanosoma cruzi.PloS one · 2013Article
- A semantic problem solving environment for integrative parasite research: identification of intervention targets for Trypanosoma cruzi.PLoS neglected tropical diseases · 2012Article
- Knockout of the dhfr-ts gene in Trypanosoma cruzi generates attenuated parasites able to confer protection against a virulent challenge.PLoS neglected tropical diseases · 2011Article
- Oral exposure to Trypanosoma cruzi elicits a systemic CD8⁺ T cell response and protection against heterotopic challenge.Infection and immunity · 2011Article
- Widespread, focal copy number variations (CNV) and whole chromosome aneuploidies in Trypanosoma cruzi strains revealed by array comparative genomic hybridization.BMC genomics · 2011Article
- A rapid, efficient and economical method for generating leishmanial gene targeting constructs.Molecular and biochemical parasitology · 2011Article
- A high-throughput cloning system for reverse genetics in Trypanosoma cruzi.BMC microbiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundTrypanosoma cruzi, a kinetoplastid protozoan parasite that causes Chagas disease, infects approximately 15 million people in Central and South America. In contrast to the substantial in silico studies of the T. cruzi genome, transcriptome, and proteome, only a few genes have been experimentally characterized and validated, mainly due to the lack of facile methods for gene manipulation needed for reverse genetic studies. Current strategies for gene disruption in T. cruzi are tedious and time consuming. In this study we have compared the conventional multi-step cloning technique with two knockout strategies that have been proven to work in other organisms, one-step-PCR- and Multisite Gateway-based systems.
resultsWhile the one-step-PCR strategy was found to be the fastest method for production of knockout constructs, it does not efficiently target genes of interest using gene-specific sequences of less than 80 nucleotides. Alternatively, the Multisite Gateway based approach is less time-consuming than conventional methods and is able to efficiently and reproducibly delete target genes.
conclusionUsing the Multisite Gateway strategy, we have rapidly produced constructs that successfully produce specific gene deletions in epimastigotes of T. cruzi. This methodology should greatly facilitate reverse genetic studies in T. cruzi.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.