ArticleInfluenza and other respiratory viruses2007
Real-time RT-PCR assays for type and subtype detection of influenza A and B viruses.
Article in Influenza and other respiratory viruses, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Trial
- Establishment and clinical applications of a portable system for capturing influenza viruses released through coughing.PloS one · 2014Trial
- Synergistic Inactivation of Airborne Viruses by Low-Concentration Ozone With High Humidity and Temperature.Microbiology and immunology · 2025Article
- Minimal influenza virus transmission from touching contaminated face masks: a laboratory study.Scientific reports · 2024Article
- Development of Aptamers for RNase Inactivation in Xtract-Free™ Sample Collection and Transport Medium.Diagnostics (Basel, Switzerland) · 2024Article
- Article
- Rapid Detection of SARS-CoV-2 Variants of Concern by Genomic Surveillance Techniques.Advances in experimental medicine and biology · 2023Article
- Article
- Article
- Circulation of influenza A and B in the Czech Republic from 2000-2001 to 2015-2016.BMC infectious diseases · 2019Article
- Growth enhancement of porcine epidemic diarrhea virus (PEDV) in Vero E6 cells expressing PEDV nucleocapsid protein.PloS one · 2019Article
- Detection of Influenza A viruses at migratory bird stopover sites in Michigan, USA.Infection ecology & epidemiology · 2018Article
- Viral respiratory infections in a nursing home: a six-month prospective study.BMC infectious diseases · 2016Article
- Avian Influenza Virus with Hemagglutinin-Neuraminidase Combination H8N8, Isolated in Russia.Genome announcements · 2014Article
- Detection of human coronaviruses in simultaneously collected stool samples and nasopharyngeal swabs from hospitalized children with acute gastroenteritis.Virology journal · 2013Article
- Sequence-specific and visual identification of the influenza virus NS gene by azobenzene-tethered bis-peptide nucleic acid.PloS one · 2013Article
- Deployable laboratory response to influenza pandemic; PCR assay field trials and comparison with reference methods.PloS one · 2011Article
- Simultaneous typing and HA/NA subtyping of influenza A and B viruses including the pandemic influenza A/H1N1 2009 by multiplex real-time RT-PCR.Journal of virological methods · 2010Article
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Authors and funding
6 authors.
Funding
Abstract
Influenza viruses type A (H3N2 and H1N1 subtypes) and B are the most prevalently circulating human influenza viruses. However, an increase in several confirmed cases of high pathogenic H5N1 in humans has raised concerns of a potential pandemic underscoring the need for rapid, point of contact detection. In this report, we describe development and evaluation of 'type,' i.e., influenza virus A and B, and 'subtype,' i.e., H1, H3, and H5, specific, single-step/reaction vessel format, real-time RT-PCR assays using total RNA from archived reference strains, shell-vial cultured and uncultured primary (throat swab/nasal wash) clinical samples. The type A and B specific assays detected all 16 influenza type A viruses and both currently circulating influenza B lineages (Yamagata and Victoria), respectively. 'Type' and 'subtype' specific assays utilize one common set of thermocycling conditions, are specific and highly sensitive (detection threshold of approximately 100 target template molecules). All clinical specimens and samples were evaluated using both the unconventional portable Ruggedized Advanced Pathogen Identification Device (RAPID) and standard laboratory bench LightCycler instruments. These potentially field-deployable assays could offer significant utility for rapid, point of care screening needs arising from a pandemic influenza outbreak.
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