ArticleBiophysical journal1989
Separation of the rotational contribution in fluorescence correlation experiments.
Article in Biophysical journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 69 citations in OpenAlex.
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- Solution-phase sample-averaged single-particle spectroscopy of quantum emitters with femtosecond resolution.Nature materials · 2024Article
- The Onset of Molecule-Spanning Dynamics in Heat Shock Protein Hsp90.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Burst-by-Burst Measurement of Rotational Diffusion at Nanosecond Resolution Reveals Hot-Brownian Motion and Single-Chain Binding.ACS nano · 2023Article
- Conformational Dynamics of Intrinsically Disordered Proteins Regulate Biomolecular Condensate Chemistry.Chemical reviews · 2022Review
- Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy.Scientific reports · 2021Article
- Semiconductor Nanocrystals for Biological Imaging and Fluorescence Spectroscopy.Advances in experimental medicine and biology · 2021Article
- Dye Encapsulation in Fluorescent Core-Shell Silica Nanoparticles as Probed by Fluorescence Correlation Spectroscopy.The journal of physical chemistry. C, Nanomaterials and interfaces · 2019Article
- Analysis of quantum rod diffusion by polarized fluorescence correlation spectroscopy.Journal of fluorescence · 2014Article
- Structural changes of yellow Cameleon domains observed by quantitative FRET analysis and polarized fluorescence correlation spectroscopy.Biophysical journal · 2008Article
- Circumvention of fluorophore photobleaching in fluorescence fluctuation experiments: a beam scanning approach.Chemphyschem : a European journal of chemical physics and physical chemistry · 2007Article
- Rotational and translational diffusion of peptide-coated CdSe/CdS/ZnS nanorods studied by fluorescence correlation spectroscopy.Journal of the American Chemical Society · 2006Article
- Pulsed interleaved excitation.Biophysical journal · 2005Article
- Fluorescence intensity and lifetime distribution analysis: toward higher accuracy in fluorescence fluctuation spectroscopy.Biophysical journal · 2002Article
- Dynamics of ANS binding to tuna apomyoglobin measured with fluorescence correlation spectroscopy.Biophysical journal · 2001Article
- Sensitivity enhancement in fluorescence correlation spectroscopy of multiple species using time-gated detection.Biophysical journal · 2000Article
- Two-dimensional fluorescence intensity distribution analysis: theory and applications.Biophysical journal · 2000Article
- The photon counting histogram in fluorescence fluctuation spectroscopy.Biophysical journal · 1999Article
- Resolution of fluorescence correlation measurements.Biophysical journal · 1999Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The theory of fluorescence correlation spectroscopy is reexamined with the aim of separating the contribution of rotational diffusion. Under constant excitation, fluorescence correlation experiments are characterized by three polarizations: one of the incident beam and two of the two photon detectors. A set of experiments of different polarizations is proposed for study. From the results of the experiments the isotropic factor of the fluorescence intensity correlation functions can be determined, which is independent of the rotational motion of the sample molecule. This function can be used to represent each fluorescence intensity correlation function as the product of the isotropic and the rotational factors. The theory is illustrated by an experiment in which rotational diffusion of porcine pancreatic lipase labeled with Texas Red was observed Texas Red is a label that allows precise fluorescence correlation experiments even in the nanosecond time range.
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