Evidence map›Paper›PMID 19431044›Full record

ArticlePreparative biochemistry & biotechnology2009

Immunoaffinity purification of the class V chitin synthase of Wangiella (Exophiala) dermatitidis.

Dariusz Abramczyk, Paul J Szaniszlo

Open access · greenAbstract read
In one paragraph

Article in Preparative biochemistry & biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Virulence · 2019
    Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Dariusz AbramczykSection of Molecular Genetics and Microbiology, School of Biological Science and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA. Dariusz.Abramczyk@manchester.ac.uk
Paul J Szaniszlo
The University of Texas at Austin · US

Funding

WANGIELLA MODEL FOR DEMATIACEOUS FUNGAL PATHOGENSR01AI033049 · NIAID · UNIVERSITY OF TEXAS AUSTIN · PI SZANISZLO, PAUL J · 1993 to 2007
$1.8M
NIAID NIH HHS AI33049NIAID NIH HHS R01 AI033049
6 · The paper itself

Abstract

The class V chitin synthase is unique because it has a myosin motor-like domain fused to its catalytic domain. The biochemical properties of this enzyme and its function remain undefined beyond the knowledge that it is the only single chitin synthase required for sustained cell growth at elevated temperatures and, consequently, virulence. This report describes our successful efforts to isolate and purify an active and soluble form of the enzyme from the cell membranes of Wangiella by using a specific polyclonal antibody. To our knowledge, this is the first purification of a single chitin synthase of a filamentous fungus.

Indexed as

Blotting, WesternCatalytic DomainChitin SynthaseChromatography, AffinityElectrophoresis, Polyacrylamide GelExophialaChitin Synthase

Identifiers

PMID19431044
PMCPMC2727359
OpenAlexW2025890552

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.