ArticlePloS one2009
The ELG1 clamp loader plays a role in sister chromatid cohesion.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 63 citations in OpenAlex.
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- Fdo1, Fkh1, Fkh2, and the Swi6-Mbp1 MBF complex regulate Mcd1 levels to impact eco1 rad61 cell growth in Saccharomyces cerevisiae.Genetics · 2024Article
- Chromatin assembly factor-1 preserves genome stability inCell stress · 2023Article
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- Review
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- Increased numbers of nucleoli in a genome-wide RNAi screen reveal proteins that link the cell cycle to RNA polymerase I transcription.Molecular biology of the cell · 2021Article
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- Eukaryotic clamp loaders and unloaders in the maintenance of genome stability.Experimental & molecular medicine · 2020Review
- PCNA promotes context-specific sister chromatid cohesion establishment separate from that of chromatin condensation.Cell cycle (Georgetown, Tex.) · 2020Article
- A genetic interaction map centered on cohesin reveals auxiliary factors involved in sister chromatid cohesion inJournal of cell science · 2020Article
- ATAD5 deficiency alters DNA damage metabolism and sensitizes cells to PARP inhibition.Nucleic acids research · 2020Article
- Article
- Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex.Nucleic acids research · 2019Article
- Identification of Elg1 interaction partners and effects on post-replication chromatin re-formation.PLoS genetics · 2018Article
- Article
- Article
- Structural studies of RFCEMBO reports · 2017Article
- Review
- Of Rings and Rods: Regulating Cohesin Entrapment of DNA to Generate Intra- and Intermolecular Tethers.PLoS genetics · 2016Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutations in the ELG1 gene of yeast lead to genomic instability, manifested in high levels of genetic recombination, chromosome loss, and gross chromosomal rearrangements. Elg1 shows similarity to the large subunit of the Replication Factor C clamp loader, and forms a RFC-like (RLC) complex in conjunction with the 4 small RFC subunits. Two additional RLCs exist in yeast: in one of them the large subunit is Ctf18, and in the other, Rad24. Ctf18 has been characterized as the RLC that functions in sister chromatid cohesion. Here we present evidence that the Elg1 RLC (but not Rad24) also plays an important role in this process. A genetic screen identified the cohesin subunit Mcd1/Scc1 and its loader Scc2 as suppressors of the synthetic lethality between elg1 and ctf4. We describe genetic interactions between ELG1 and genes encoding cohesin subunits and their accessory proteins. We also show that defects in Elg1 lead to higher precocious sister chromatid separation, and that Ctf18 and Elg1 affect cohesion via a joint pathway. Finally, we localize both Ctf18 and Elg1 to chromatin and show that Elg1 plays a role in the recruitment of Ctf18. Our results suggest that Elg1, Ctf4, and Ctf18 may coordinate the relative movement of the replication fork with respect to the cohesin ring.
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