ArticleJournal of biosciences2009
Replica-moulded polydimethylsiloxane culture vessel lids attenuate osmotic drift in long-term cell cultures.
Article in Journal of biosciences, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
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- The Profile of Network Spontaneous Activity and Functional Organization Interplay in Hierarchically Connected Modular Neural Networks In Vitro.Micromachines · 2024Article
- Microfluidic Bi-Layer Platform to Study Functional Interaction between Co-Cultured Neural Networks with Unidirectional Synaptic Connectivity.Micromachines · 2023Article
- Human neural network activity reacts to gravity changesFrontiers in neuroscience · 2023Article
- Tracking connectivity maps in human stem cell-derived neuronal networks by holographic optogenetics.Life science alliance · 2022Article
- Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.iScience · 2021Article
- Transportable system enabling multiple irradiation studies under simultaneous hypoxia in vitro.Radiation oncology (London, England) · 2018Article
- Stimulation triggers endogenous activity patterns in cultured cortical networks.Scientific reports · 2017Article
- A multielectrode array microchannel platform reveals both transient and slow changes in axonal conduction velocity.Scientific reports · 2017Article
- Burst predicting neurons survive an in vitro glutamate injury model of cerebral ischemia.Scientific reports · 2015Article
- Incubator-independent cell-culture perfusion platform for continuous long-term microelectrode array electrophysiology and time-lapse imaging.Royal Society open science · 2015Article
- Upward synaptic scaling is dependent on neurotransmission rather than spiking.Nature communications · 2015Article
- Article
- Long-term optical stimulation of channelrhodopsin-expressing neurons to study network plasticity.Frontiers in molecular neuroscience · 2013Article
- Effects of antiepileptic drugs on hippocampal neurons coupled to micro-electrode arrays.Frontiers in neuroengineering · 2013Article
- Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses.The Journal of biological chemistry · 2011Article
- Experimental Investigation on Spontaneously Active Hippocampal Cultures Recorded by Means of High-Density MEAs: Analysis of the Spatial Resolution Effects.Frontiers in neuroengineering · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An imbalance in medium osmolarity is a determinant that affects cell culture longevity. Even in humidified incubators, evaporation of water leads to a gradual increase in osmolarity over time. We present a simple replica-moulding strategy for producing self-sealing lids adaptable to standard, small-size cell-culture vessels. They are made of polydimethylsiloxane (PDMS), a flexible, transparent and biocompatible material, which is gas-permeable but largely impermeable to water. Keeping cell cultures in a humidified 5% CO2 incubator at 37 degrees C, medium osmolarity increased by +6.86 mosmol/kg/day in standard 35 mm Petri dishes, while PDMS lids attenuated its rise by a factor of four to changes of +1.72 mosmol/kg/ day. Depending on the lid membrane thickness,pH drifts at ambient CO2 levels were attenuated by a factor of 4 to 9. Comparative evaporation studies at temperatures below 60 degrees C yielded a 10-fold reduced water vapour flux of 1.75 g/day/ dm 2 through PDMS lids as compared with 18.69 g/day/dm 2 with conventional Petri dishes. Using such PDMS lids,about 2/3 of the cell cultures grew longer than 30 days in vitro. Among these,the average survival time was 69 days with the longest survival being 284 days under otherwise conventional cell culture conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.