ArticleEukaryotic cell2009
Analysis of flagellar phosphoproteins from Chlamydomonas reinhardtii.
Article in Eukaryotic cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Who cites it
31 citing papers in PubMed, 62 citations in OpenAlex.
- Multiomics studies reveal how ambient temperature changes govern cellular responses of Chlamydomonas.The Plant cell · 2026Article
- Advances in ciliary proteomics - towards cracking the hidden proteome code of cilia.Journal of cell science · 2025Review
- Cilia.Pro database of ciliary proteins from vertebrates,Molecular biology of the cell · 2025Article
- Article
- CilioGenics: an integrated method and database for predicting novel ciliary genes.Nucleic acids research · 2024Article
- The methylome of motile cilia.Molecular biology of the cell · 2024Article
- A deep learning based approach for prediction of Chlamydomonas reinhardtii phosphorylation sites.Scientific reports · 2021Article
- Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link.PLoS genetics · 2021Article
- A Musashi Splice Variant and Its Interaction Partners Influence Temperature Acclimation inPlant physiology · 2018Article
- Identification of a flagellar protein implicated in the gravitaxis in the flagellate Euglena gracilis.Scientific reports · 2018Article
- Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways.Developmental cell · 2017Article
- Alcohol-induced ciliary dysfunction targets the outer dynein arm.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- The global phosphoproteome of Chlamydomonas reinhardtii reveals complex organellar phosphorylation in the flagella and thylakoid membrane.Molecular & cellular proteomics : MCP · 2014Article
- Insights into the structure and function of ciliary and flagellar doublet microtubules: tektins, Ca2+-binding proteins, and stable protofilaments.The Journal of biological chemistry · 2014Article
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- Article
- The microtubule affinity regulating kinase MARK4 promotes axoneme extension during early ciliogenesis.The Journal of cell biology · 2013Article
- Regulation of flagellar biogenesis by a calcium dependent protein kinase in Chlamydomonas reinhardtii.PloS one · 2013Article
- The eukaryotic flagellum makes the day: novel and unforeseen roles uncovered after post-genomics and proteomics data.Current protein & peptide science · 2012Review
- Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cilia and flagella are cell organelles that are highly conserved throughout evolution. For many years, the green biflagellate alga Chlamydomonas reinhardtii has served as a model for examination of the structure and function of its flagella, which are similar to certain mammalian cilia. Proteome analysis revealed the presence of several kinases and protein phosphatases in these organelles. Reversible protein phosphorylation can control ciliary beating, motility, signaling, length, and assembly. Despite the importance of this posttranslational modification, the identities of many ciliary phosphoproteins and knowledge about their in vivo phosphorylation sites are still missing. Here we used immobilized metal affinity chromatography to enrich phosphopeptides from purified flagella and analyzed them by mass spectrometry. One hundred forty-one phosphorylated peptides were identified, belonging to 32 flagellar proteins. Thereby, 126 in vivo phosphorylation sites were determined. The flagellar phosphoproteome includes different structural and motor proteins, kinases, proteins with protein interaction domains, and many proteins whose functions are still unknown. In several cases, a dynamic phosphorylation pattern and clustering of phosphorylation sites were found, indicating a complex physiological status and specific control by reversible protein phosphorylation in the flagellum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.