ArticleJournal of bacteriology2009
Correct timing of dnaA transcription and initiation of DNA replication requires trans translation.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- An antisense RNA regulates production of DnaA and affects sporulation in Bacillus subtilis.PLoS genetics · 2025Article
- Article
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- A nascent polypeptide sequence modulates DnaA translation elongation in response to nutrient availability.eLife · 2021Article
- Transcriptional Activity of the Bacterial Replication Initiator DnaA.Frontiers in microbiology · 2021Review
- The Protein Quality Control Network inFrontiers in molecular biosciences · 2021Review
- Multilayered control of chromosome replication inBiochemical Society transactions · 2019Review
- Non-coding RNAs Potentially Controlling Cell Cycle in the ModelFrontiers in genetics · 2018Article
- Nutritional Control of DNA Replication Initiation through the Proteolysis and Regulated Translation of DnaA.PLoS genetics · 2015Article
- The coding and noncoding architecture of the Caulobacter crescentus genome.PLoS genetics · 2014Article
- Computational and genetic reduction of a cell cycle to its simplest, primordial components.PLoS biology · 2013Article
- The tmRNA ribosome-rescue system.Advances in protein chemistry and structural biology · 2012Review
- Bacterial small RNA regulators: versatile roles and rapidly evolving variations.Cold Spring Harbor perspectives in biology · 2011Review
- Modularity of the bacterial cell cycle enables independent spatial and temporal control of DNA replication.Current biology : CB · 2011Article
- Regulation of the activity of the dual-function DnaA protein in Caulobacter crescentus.PloS one · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The trans translation pathway for protein tagging and ribosome release has been found in all bacteria and is required for proliferation and differentiation in many systems. Caulobacter crescentus mutants that lack the trans translation pathway have a defect in the cell cycle and do not initiate DNA replication at the correct time. To determine the molecular basis for this phenotype, effects on events known to be important for initiation of DNA replication were investigated. In the absence of trans translation, transcription from the dnaA promoter and an origin-proximal promoter involved in replication initiation is delayed. Characterization of the dnaA promoter revealed two cis-acting elements that have dramatic effects on dnaA gene expression. A 5' leader sequence in dnaA mRNA represses gene expression by >15-fold but does not affect the timing of dnaA expression. The second cis-acting element, a sequence upstream of the -35 region, affects both the amount of dnaA transcription and the timing of transcription in response to trans translation. Mutations in this promoter element eliminate the transcription delay and partially suppress the DNA replication phenotype in mutants lacking trans translation activity. These results suggest that the trans translation capacity of the cell is sensed through the dnaA promoter to control the timing of DNA replication initiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.