ArticlePlant physiology2009
Site-specific integration of transgenes in soybean via recombinase-mediated DNA cassette exchange.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed, 84 citations in OpenAlex.
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Copy number variations at theFrontiers in plant science · 2024Article
- Identification of neutral genome integration sites with high expression and high integration efficiency inSynthetic and systems biotechnology · 2023Article
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- CRISPR/LbCas12a-Mediated Genome Editing in Soybean.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Target Lines forInternational journal of molecular sciences · 2022Article
- Split-Cre mediated deletion of DNA no longer needed after site-specific integration in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Target lines for recombinase-mediated gene stacking in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Site-Specific Sequence Exchange Between Homologous and Non-homologous Chromosomes.Frontiers in plant science · 2022Article
- Artificial chromosome technology and its potential application in plants.Frontiers in plant science · 2022Review
- Targeted DNA insertion in plants.Proceedings of the National Academy of Sciences of the United States of America · 2021Review
- Recombinase-mediated integration of a multigene cassette in rice leads to stable expression and inheritance of the stacked locus.Plant direct · 2020Article
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- Complex Trait Loci in Maize Enabled by CRISPR-Cas9 Mediated Gene Insertion.Frontiers in plant science · 2020Article
- High efficiency Agrobacterium-mediated site-specific gene integration in maize utilizing the FLP-FRT recombination system.Plant biotechnology journal · 2019Article
- Generation of a selectable marker free, highly expressed single copy locus as landing pad for transgene stacking in sugarcane.Plant molecular biology · 2019Article
- Zinc finger nuclease-mediated targeting of multiple transgenes to an endogenous soybean genomic locus via non-homologous end joining.Plant biotechnology journal · 2019Article
- Uniform Expression and Relatively Small Position Effects Characterize Sister Transformants in Maize and Soybean.Frontiers in plant science · 2019Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- An Improved Variant of Soybean Type 1 Diacylglycerol Acyltransferase Increases the Oil Content and Decreases the Soluble Carbohydrate Content of Soybeans.Plant physiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A targeting method to insert genes at a previously characterized genetic locus to make plant transformation and transgene expression predictable is highly desirable for plant biotechnology. We report the successful targeting of transgenes to predefined soybean (Glycine max) genome sites using the yeast FLP-FRT recombination system. First, a target DNA containing a pair of incompatible FRT sites flanking a selection gene was introduced in soybean by standard biolistic transformation. Transgenic events containing a single copy of the target were retransformed with a donor DNA, which contained the same pair of FRT sites flanking a different selection gene, and a FLP expression DNA. Precise DNA cassette exchange was achieved between the target and donor DNA via recombinase-mediated cassette exchange, so that the donor DNA was introduced at the locus previously occupied by the target DNA. The introduced donor genes expressed normally and segregated according to Mendelian laws.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.