ArticleMolecular and biochemical parasitology2009
Efficient genotyping of Schistosoma mansoni miracidia following whole genome amplification.
Article in Molecular and biochemical parasitology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Abundant genetic variation is retained in many laboratory schistosome populations.PLoS pathogens · 2025Article
- Integrating genomic and epidemiologic data to accelerate progress toward schistosomiasis elimination.eLife · 2022Review
- Identification of genetic variations inThe Journal of international medical research · 2019Article
- Population Structure and Dynamics of Helminthic Infection: Schistosomiasis.Microbiology spectrum · 2019Review
- Whole genome amplification and exome sequencing of archived schistosome miracidia.Parasitology · 2018Article
- The influence of related and unrelated co-infections on parasite dynamics and virulence.Oecologia · 2018Article
- Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia.PLoS neglected tropical diseases · 2017Article
- Detecting genotyping errors at Schistosoma japonicum microsatellites with pedigree information.Parasites & vectors · 2015Article
- Characterisation of a novel panel of polymorphic microsatellite loci for the liver fluke, Fasciola hepatica, using a next generation sequencing approach.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2015Article
- Efficient linkage mapping using exome capture and extreme QTL in schistosome parasites.BMC genomics · 2014Article
- Approaches to genotyping individual miracidia of Schistosoma japonicum.Parasitology research · 2013Article
- Significant variance in genetic diversity among populations of Schistosoma haematobium detected using microsatellite DNA loci from a genome-wide database.Parasites & vectors · 2013Article
- Molecular assessment of trematode co-infection and intraspecific competition in molluscan intermediate hosts.Molecular and biochemical parasitology · 2013Article
- Schistosomiasis collection at NHM (SCAN).Parasites & vectors · 2012Article
- Whole-genome sequence of Schistosoma haematobium.Nature genetics · 2012Article
- Applying evolutionary genetics to schistosome epidemiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2010Review
- In vitro manipulation of gene expression in larval Schistosoma: a model for postgenomic approaches in Trematoda.Parasitology · 2010Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Small parasites and larval stages pose a problem for molecular analyses because limited amounts of DNA template are available. Isothermal methods for faithfully copying DNA have the potential to revolutionize studies of such organisms. We evaluated the fidelity of multiple displacement amplification (MDA) for amplifying DNA extracted from a single miracidium of Schistosoma mansoni. To do this we genotyped DNA extracted from 28 F1 miracidia following MDA using 56 microsatellite markers. Because these miracidia were obtained from a cross between a male and female worm of known genotypes, we were able to predict the alleles present in the progeny and quantify the genotyping error rate. We found just 8/1568 genotypes deviated from Mendelian expectations. Furthermore, because 1 of these resulted from a genuine mutation, the error rate due to MDA is 7/1568 (0.45%). We conclude that many hundreds of microsatellites or other genetic markers can be accurately genotyped from a single miracidium using this method, greatly expanding the scope of population genetic, epidemiological and evolutionary studies on this parasite.
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