ArticleMethods in enzymology2009
Functional characterization of phosphorylation sites in dynamin-related protein 1.
Article in Methods in enzymology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 55 citations in OpenAlex.
- PHLPP1 regulates region-specific astroglial mitochondrial fission in response to oxidative stress in the male rat hippocampus.Scientific reports · 2025Article
- The E3-ligase Siah2 activates mitochondrial quality control in neurons to maintain energy metabolism during ischemic brain tolerance.Cell death & disease · 2025Article
- Reciprocal regulation of oxidative stress and mitochondrial fission augments parvalbumin downregulation through CDK5-DRP1- and GPx1-NF-κB signaling pathways.Cell death & disease · 2024Article
- Allosteric control of dynamin-related protein 1 through a disordered C-terminal Short Linear Motif.Nature communications · 2024Article
- Mitochondrial Dynamics in Pulmonary Hypertension.Biomedicines · 2023Review
- Mitochondrial heterogeneity in diseases.Signal transduction and targeted therapy · 2023Review
- Discovery of Potent Allosteric DRP1 Inhibitors by Disrupting Protein-Protein Interaction with MiD49.ACS medicinal chemistry letters · 2023Article
- Mitochondrial morphology controls fatty acid utilization by changing CPT1 sensitivity to malonyl-CoA.The EMBO journal · 2023Article
- EGCG Attenuates CA1 Neuronal Death by Regulating GPx1, NF-κB S536 Phosphorylation and Mitochondrial Dynamics in the Rat Hippocampus following Status Epilepticus.Antioxidants (Basel, Switzerland) · 2023Article
- CDDO-Me Abrogates Aberrant Mitochondrial Elongation in Clasmatodendritic Degeneration by Regulating NF-κB-PDI-Mediated S-Nitrosylation of DRP1.International journal of molecular sciences · 2023Article
- Article
- CDDO-Me Attenuates CA1 Neuronal Death by Facilitating RalBP1-Mediated Mitochondrial Fission and 4-HNE Efflux in the Rat Hippocampus Following Status Epilepticus.Antioxidants (Basel, Switzerland) · 2022Article
- CDDO-Me Attenuates Clasmatodendrosis in CA1 Astrocyte by Inhibiting HSP25-AKT Mediated DRP1-S637 Phosphorylation in Chronic Epilepsy Rats.International journal of molecular sciences · 2022Article
- ERK/Drp1-dependent mitochondrial fission contributes to HMGB1-induced autophagy in pulmonary arterial hypertension.Cell proliferation · 2021Article
- LONP1 Regulates Mitochondrial Accumulations of HMGB1 and Caspase-3 in CA1 and PV Neurons Following Status Epilepticus.International journal of molecular sciences · 2021Article
- High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Mito Hacker: a set of tools to enable high-throughput analysis of mitochondrial network morphology.Scientific reports · 2020Article
- Knocking-out the Siah2 E3 ubiquitin ligase prevents mitochondrial NCX3 degradation, regulates mitochondrial fission and fusion, and restores mitochondrial function in hypoxic neurons.Cell communication and signaling : CCS · 2020Article
- Loss of MCU prevents mitochondrial fusion in GScience signaling · 2019Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Dynamin-related protein 1 is member of the dynamin-family of large GTPases. Similar to the endocytosis motor dynamin, Drp1 uses GTP hydrolysis to power constriction of the outer mitochondrial membrane and ultimately mitochondrial division. Dynamin phosphorylation in its unique C-terminal proline-rich domain interferes with binding of accessory proteins that induce membrane curvature and inhibits clathrin-mediated endocytosis. Evidence within the last few years indicates that Drp1 is also regulated by the phosphorylation/dephosphorylation cycle. Drp1 regulation is complex, in that both inhibitory and activating phosphorylations have been described that lead to, respectively, mitochondrial elongation and shortening. In this chapter, we describe methods for the identification and functional characterization of Drp1 phosphorylation sites. Among these methods is replacement of the endogenous protein by phosphorylation-site mutant Drp1 via combined shRNA and RNAi-resistant cDNA expression from the same plasmid. We also discuss primary astrocyte cultures as a model for regulation of cell death and mitochondrial morphology by Drp1 and present ImageJ macro source code for unbiased quantification of mitochondrial shape changes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.