ArticleBMC research notes2009
Differential gene expression signatures for cell wall integrity found in chitin synthase II (chs2Delta) and myosin II (myo1Delta) deficient cytokinesis mutants of Saccharomyces cerevisiae.
Article in BMC research notes, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 7 citations in OpenAlex.
- CgEnd3 Regulates Endocytosis, Appressorium Formation, and Virulence in the Poplar Anthracnose FungusInternational journal of molecular sciences · 2021Article
- Protein Interactions of the Mechanosensory Proteins Wsc2 and Wsc3 for Stress Resistance inG3 (Bethesda, Md.) · 2020Article
- Cell wall stress induces alternative fungal cytokinesis and septation strategies.Journal of cell science · 2013Article
- Functional and genetic interactions of TOR in the budding yeast Saccharomyces cerevisiae with myosin type II-deficiency (myo1Δ).BMC cell biology · 2012Article
- Article
- Boric Acid Disturbs Cell Wall Synthesis in Saccharomyces cerevisiae.International journal of microbiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMyosin II-dependent contraction of the cytokinetic ring and primary septum formation by chitin synthase II are interdependent processes during cytokinesis in Saccharomyces cerevisiae. Hence, null mutants of myosin II (myo1Delta) and chitin synthase II (chs2Delta) share multiple morphological and molecular phenotypes. To understand the nature of their interdependent functions, we will seek to identify genes undergoing transcriptional regulation in chs2Delta strains and to establish a transcription signature profile for comparison with myo1Delta strains.
resultsA total of 467 genes were commonly regulated between myo1Delta and chs2Delta mutant strains (p <or= 0.01). Common regulated biological process categories identified by Gene Set Enrichment Analysis (GSEA) in both gene expression profiles were: protein biosynthesis, RNA processing, and stress response. Expression of 17/20 genes in the main transcriptional fingerprint for cell wall stress was confirmed in the chs2Delta strain versus 5/20 for the myo1Delta strain. One of these genes, SLT2/MPK1, was up-regulated in both strains and both strains accumulated the hyperphosphorylated form of Slt2p thereby confirming that the PKC1 cell wall integrity pathway (CWIP) was activated by both mutations. The SLT2/MPK1 gene, essential for myo1Delta strains, was not required in the chs2Delta strain.
conclusionComparison of the chs2Delta and myo1Delta gene expression profiles revealed similarities in the biological process categories that respond to the chs2Delta and myo1Delta gene mutations. This supports the view that these mutations affect a common function in cytokinesis. Despite their similarities, these mutants exhibited significant differences in expression of the main transcriptional fingerprint for cell wall stress and their requirement of the CWIP for survival.
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Registered trials
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