ArticleJournal of cellular and molecular medicine2010
Dedifferentiation derived cells exhibit phenotypic and functional characteristics of epidermal stem cells.
Article in Journal of cellular and molecular medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Mechanisms of diabetic foot ulceration: A review.Journal of diabetes · 2023Review
- Cellular rejuvenation: molecular mechanisms and potential therapeutic interventions for diseases.Signal transduction and targeted therapy · 2023Review
- Identification of Key Functional Gene Signatures Indicative of Dedifferentiation in Papillary Thyroid Cancer.Frontiers in oncology · 2021Article
- A Dedifferentiation Strategy to Enhance the Osteogenic Potential of Dental Derived Stem Cells.Frontiers in cell and developmental biology · 2021Article
- Alternating Differentiation and Dedifferentiation between Mature Osteoblasts and Osteocytes.Scientific reports · 2019Article
- Spontaneous evolution of human skin fibroblasts into wound-healing keratinocyte-like cells.Theranostics · 2019Article
- Regenerative medicine in China: main progress in different fields.Military Medical Research · 2016Review
- Bulk pancreatic cancer cells can convert into cancer stem cells(CSCs) in vitro and 2 compounds can target these CSCs.Cell cycle (Georgetown, Tex.) · 2016Article
- Overexpression of cyclin D1 induces the reprogramming of differentiated epidermal cells into stem cell-like cells.Cell cycle (Georgetown, Tex.) · 2016Article
- Effect of mixed transplantation of autologous and allogeneic microskin grafts on wound healing in a rat model of acute skin defect.PloS one · 2014Article
- Model on cell movement, growth, differentiation and de-differentiation: reaction-diffusion equation and wave propagation.The European physical journal. E, Soft matter · 2013Article
- HGF accelerates wound healing by promoting the dedifferentiation of epidermal cells through β1-integrin/ILK pathway.BioMed research international · 2013Article
- Localization of human mesenchymal stem cells from umbilical cord blood and their role in repair of diabetic foot ulcers in rats.International journal of biological sciences · 2013Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differentiated epidermal cells can dedifferentiate into stem cells or stem cell-like cells in vivo. In this study, we report the isolation and characterization of dedifferentiation-derived cells. Epidermal sheets eliminated of basal stem cells were transplanted onto the skin wounds in 47 nude athymic (BALB/c-nu/nu) mice. After 5 days, cells negative for CK10 but positive for CK19 and beta1-integrin emerged at the wound-neighbouring side of the epidermal sheets. Furthermore, the percentages of CK19 and beta1-integrin+ cells detected by flow cytometric analysis were increased after grafting (P < 0.01) and CK10+ cells in grafted sheets decreased (P < 0.01). Then we isolated these cells on the basis of rapid adhesion to type IV collagen and found that there were 4.56% adhering cells (dedifferentiation-derived cells) in the grafting group within 10 min. The in vitro phenotypic assays showed that the expressions of CK19, beta1-integrin, Oct4 and Nanog in dedifferentiation-derived cells were remarkably higher than those in the control group (differentiated epidermal cells) (P < 0.01). In addition, the results of the functional investigation of dedifferentiation-derived cells demonstrated: (1) the numbers of colonies consisting of 5-10 cells and greater than 10 cells were increased 5.9-fold and 6.7-fold, respectively, as compared with that in the control (P < 0.01); (2) more cells were in S phase and G2/M phase of the cell cycle (proliferation index values were 21.02% in control group, 45.08% in group of dedifferentiation); (3) the total days of culture (28 days versus 130 days), the passage number of cells (3 passages versus 20 passages) and assumptive total cell output (1 x 10(5) cells versus 1 x 10(12) cells) were all significantly increased and (4) dedifferentiation-derived cells, as well as epidermal stem cells, were capable of regenerating a skin equivalent, but differentiated epidermal cells could not. These results suggested that the characteristics of dedifferentiation-derived cells cultured in vitro were similar to epidermal stem cells. This study may also offer a new approach to yield epidermal stem cells for wound repair and regeneration.
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