ArticleClinical orthopaedics and related research2009
Treating human meniscal fibrochondrocytes with hIGF-1 gene by liposome.
Article in Clinical orthopaedics and related research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Biochemical Stimulus-Based Strategies for Meniscus Tissue Engineering and Regeneration.BioMed research international · 2018Review
- Advances in combining gene therapy with cell and tissue engineering-based approaches to enhance healing of the meniscus.Osteoarthritis and cartilage · 2016Review
- Comparison of hIGF-1 gene transfection to the hBMSCs and human meniscal fibrochondrocytes.Medical science monitor : international medical journal of experimental and clinical research · 2015Article
- Tissue-engineering strategies to repair joint tissue in osteoarthritis: nonviral gene-transfer approaches.Current rheumatology reports · 2014Review
- The meniscal healing process.Muscles, ligaments and tendons journal · 2012Article
- Gene Therapy for Cartilage Repair.Cartilage · 2011Review
- Clinical potential and challenges of using genetically modified cells for articular cartilage repair.Croatian medical journal · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The menisci are intraarticular fibrocartilaginous structures essential to the normal function of the knee that lack the ability to self-repair. Human meniscal fibrochondrocytes may respond to beneficial genes like human insulin growth factor-1 (hIGF-1) and the meniscal cell may be a feasible donor for gene therapy. To explore this possibility, we amplified the hIGF-1 gene sequence in full length and cloned it into a bicistronic plasmid. This gene was then transfected into cultured human meniscal fibrochondrocytes by the liposome FuGene 6. Green fluorescence was expressed in part of the cells 6 hours after transfection and increased gradually, with a peak concentration of the hIGF-1 in the supernatants to 22.68 ng/mL 56 hours after transfection. Phenotypes of some cells changed and the proliferation accelerated after transfection. Flow cytometry analysis demonstrated upregulation of cell numbers in the G2 and S stages after hIGF-1 gene introduction. We conclude the hIGF-1 gene can be transfected into the human meniscal cell efficiently by liposome and it causes accelerated proliferation and differentiation. Within 10 days after transfection, the cytokine appears to be secreted into supernatants with the bioactivity and promotes the proliferation of the NIH 3T3 cell line.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.