ArticlePLoS genetics2009
Microdissection of shoot meristem functional domains.
Article in PLoS genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 83 citations in OpenAlex.
- Unlocking grass leaf development: foundations for tunable cereal design.The New phytologist · 2025Review
- Identification of candidate genes for leaf size by QTL mapping and transcriptome sequencing in Brassica napus L.BMC genomics · 2025Article
- A dynamic regulome of shoot-apical-meristem-related homeobox transcription factors modulates plant architecture in maize.Genome biology · 2024Article
- Laser-Assisted Microdissection and High-Throughput RNA Sequencing of theBio-protocol · 2024Article
- Genome-wide association studies from spoken phenotypic descriptions: a proof of concept from maize field studies.G3 (Bethesda, Md.) · 2024Article
- An in situ sequencing approach maps PLASTOCHRON1 at the boundary between indeterminate and determinate cells.Plant physiology · 2022Article
- Genome-wide analysis of spatiotemporal expression patterns during rice leaf development.BMC genomics · 2021Article
- Developmental genetics of maize vegetative shoot architecture.Molecular breeding : new strategies in plant improvement · 2021Article
- Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based Method.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Molecular diversity and selective sweeps in maize inbred lines adapted to African highlands.Scientific reports · 2019Article
- Transcriptome analysis highlights nuclear control of chloroplast development in the shoot apex.Scientific reports · 2018Article
- Identification and Fine-Mapping of a Major Maize Leaf Width QTL in a Re-sequenced Large Recombinant Inbred Lines Population.Frontiers in plant science · 2018Article
- MaizeThe Plant cell · 2017Article
- Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development.Journal of visualized experiments : JoVE · 2017Article
- Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling.Nature protocols · 2016Article
- Differential regulation of meristem size, morphology and organization by the ERECTA, CLAVATA and class III HD-ZIP pathways.Development (Cambridge, England) · 2016Article
- Combined Chlorophyll Fluorescence and Transcriptomic Analysis Identifies the P3/P4 Transition as a Key Stage in Rice Leaf Photosynthetic Development.Plant physiology · 2016Article
- An Efficient LCM-Based Method for Tissue Specific Expression Analysis of Genes and miRNAs.Scientific reports · 2016Article
- Transcriptome dynamics of developing maize leaves and genomewide prediction of cis elements and their cognate transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Laser assisted microdissection, an efficient technique to understand tissue specific gene expression patterns and functional genomics in plants.Molecular biotechnology · 2015Review
Corrections and comments
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Authors and funding
17 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The shoot apical meristem (SAM) maintains a pool of indeterminate cells within the SAM proper, while lateral organs are initiated from the SAM periphery. Laser microdissection-microarray technology was used to compare transcriptional profiles within these SAM domains to identify novel maize genes that function during leaf development. Nine hundred and sixty-two differentially expressed maize genes were detected; control genes known to be upregulated in the initiating leaf (P0/P1) or in the SAM proper verified the precision of the microdissections. Genes involved in cell division/growth, cell wall biosynthesis, chromatin remodeling, RNA binding, and translation are especially upregulated in initiating leaves, whereas genes functioning during protein fate and DNA repair are more abundant in the SAM proper. In situ hybridization analyses confirmed the expression patterns of six previously uncharacterized maize genes upregulated in the P0/P1. P0/P1-upregulated genes that were also shown to be downregulated in leaf-arrested shoots treated with an auxin transport inhibitor are especially implicated to function during early events in maize leaf initiation. Reverse genetic analyses of asceapen1 (asc1), a maize D4-cyclin gene upregulated in the P0/P1, revealed novel leaf phenotypes, less genetic redundancy, and expanded D4-CYCLIN function during maize shoot development as compared to Arabidopsis. These analyses generated a unique SAM domain-specific database that provides new insight into SAM function and a useful platform for reverse genetic analyses of shoot development in maize.
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