ArticleGenome research2009
Mapping epigenetic mutations in fission yeast using whole-genome next-generation sequencing.
Article in Genome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed.
- Clr4Nature communications · 2024Article
- Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins.Open biology · 2021Article
- Article
- MutantHuntWGS: A Pipeline for IdentifyingG3 (Bethesda, Md.) · 2020Article
- Nitrogen starvation reveals the mitotic potential of mutants in the S/MAPK pathways.Nature communications · 2020Article
- Combining next-generation sequencing and progeny testing for rapid identification of induced recessive and dominant mutations in maize MThe Plant journal : for cell and molecular biology · 2019Article
- Whole-Genome Sequencing Identifies a Rice Grain Shape Mutant, gs9-1.Rice (New York, N.Y.) · 2019Article
- Dependency of Heterochromatin Domains on Replication Factors.G3 (Bethesda, Md.) · 2018Article
- Article
- RNA interference is essential for cellular quiescence.Science (New York, N.Y.) · 2016Article
- A better way to discover gene function in the social amoeba Dictyostelium discoideum.Genome research · 2016Article
- Mapping Challenging Mutations by Whole-Genome Sequencing.G3 (Bethesda, Md.) · 2016Article
- Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype.BMC genomics · 2015Article
- Fast forward genetics to identify mutations causing a high light tolerant phenotype in Chlamydomonas reinhardtii by whole-genome-sequencing.BMC genomics · 2015Article
- Using next-generation sequencing to isolate mutant genes from forward genetic screens.Nature reviews. Genetics · 2014Review
- A genetic screen based on in vivo RNA imaging reveals centrosome-independent mechanisms for localizing gurken transcripts in Drosophila.G3 (Bethesda, Md.) · 2014Article
- Forward genetics by genome sequencing reveals that rapid cyanide release deters insect herbivory of Sorghum bicolor.Genetics · 2013Article
- Partitioning of the nuclear and mitochondrial tRNA 3'-end processing activities between two different proteins in Schizosaccharomyces pombe.The Journal of biological chemistry · 2013Article
- Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing.Development (Cambridge, England) · 2012Article
- Impaired coenzyme A synthesis in fission yeast causes defective mitosis, quiescence-exit failure, histone hypoacetylation and fragile DNA.Open biology · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Fission yeast is an important model for epigenetic studies due to the ease with which genetic mutants can be isolated. However, it can be difficult to complement epigenetic phenotypes with genomic libraries in order to identify the genes responsible. This is because epigenetic phenotypes are typically unstable, and can prohibit complementation if silencing cannot be reestablished. Here we have resequenced the fission yeast genome following mutagenesis to readily identify a novel mutant involved in heterochromatic silencing. Candidate genes were identified as functional single base changes linked to the mutation, which were then reconstituted in a wild-type strain to recapitulate the mutant phenotype. By this procedure we identified a weak allele of ubc4, which encodes an essential E2 ubiquitin ligase, as responsible for the swi*603 mutant phenotype. In combination with a large collection of mutants and suppressor plasmids, next-generation genomic resequencing promises to dramatically enhance the power of yeast genetics, permitting the isolation of subtle alleles of essential genes, alleles with quantitative effects, and enhancers and suppressors of heterochromatic silencing.
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