ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2009
Pancreatic beta cells and islets take up thiamin by a regulated carrier-mediated process: studies using mice and human pancreatic preparations.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Effect of thiamine supplementation on glycaemic outcomes in adults with type 2 diabetes: a systematic review and meta-analysis.BMJ open · 2022Pooled it
- Screening potential diagnostic biomarkers for PLA2R‑associated idiopathic membranous nephropathy by WGCNA analysis and LASSO algorithm.Renal failure · 2025Article
- Effects of dietary vitamins on obesity-related metabolic parameters.Journal of nutritional science · 2023Review
- Role of gut commensal bacteria in juvenile developmental growth of the host: insights fromAnimal cells and systems · 2023Review
- U-shaped association between dietary thiamine intake and new-onset diabetes: a nationwide cohort study.QJM : monthly journal of the Association of Physicians · 2022Article
- The role of pancreas to improve hyperglycemia in STZ-induced diabetic rats by thiamine disulfide.Nutrition & diabetes · 2022Article
- The Effects of Genetic Mutations and Drugs on the Activity of the Thiamine Transporter, SLC19A2.The AAPS journal · 2021Article
- Association between levels of thiamine intake, diabetes, cardiovascular diseases and depression in Korea: a national cross-sectional study.Journal of nutritional science · 2021Article
- The Relevance of Thiamine Evaluation in a Practical Setting.Nutrients · 2020Article
- Loss-of-Function Mutation in Thiamine Transporter 1 in a Family With Autosomal Dominant Diabetes.Diabetes · 2019Article
- Pharmacogenomics in diabetes: outcomes of thiamine therapy in TRMA syndrome.Diabetologia · 2018Observational
- Adaptive regulation of pancreatic acinar mitochondrial thiamin pyrophosphate uptake process: possible involvement of epigenetic mechanism(s).American journal of physiology. Gastrointestinal and liver physiology · 2017Article
- Thiamine Deficiency in Tropical Pediatrics: New Insights into a Neglected but Vital Metabolic Challenge.Frontiers in nutrition · 2016Review
- Chronic Nicotine Exposure In Vivo and In Vitro Inhibits Vitamin B1 (Thiamin) Uptake by Pancreatic Acinar Cells.PloS one · 2015Article
- Biotin uptake by mouse and human pancreatic beta cells/islets: a regulated, lipopolysaccharide-sensitive carrier-mediated process.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Mechanisms involved in the inhibitory effect of chronic alcohol exposure on pancreatic acinar thiamin uptake.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- Recent advances in transport of water-soluble vitamins in organs of the digestive system: a focus on the colon and the pancreas.American journal of physiology. Gastrointestinal and liver physiology · 2013Review
- Article
- Mechanism and regulation of vitamin B2 (riboflavin) uptake by mouse and human pancreatic β-cells/islets: physiological and molecular aspects.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- The impact of thiamine treatment in the diabetes mellitus.Journal of clinical medicine research · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Thiamin is essential for the normal function of the endocrine pancreas, but very little is known about uptake mechanism(s) and regulation by beta cells. We addressed these issues using mouse-derived pancreatic beta-TC-6 cells, and freshly isolated primary mouse and human pancreatic islets. Results showed that thiamin uptake by beta-TC-6 cells involves a pH (but not Na+)-dependent carrier-mediated process that is saturable at both the nanomolar (apparent K(m) = 37.17 +/- 9.9 nM) and micromolar (apparent K(m) = 3.26 +/- 0.86 microM) ranges, cis-inhibited by thiamin structural analogs, and trans-stimulated by unlabeled thiamin. Involvement of carrier-mediated process was also confirmed in primary mouse and human pancreatic islets. Both THTR-1 and THTR-2 were found to be expressed in these mouse and human pancreatic preparations. Maintaining beta-TC-6 cells in the presence of a high level of thiamin led to a significant (P < 0.01) decrease in thiamin uptake, which was associated with a significant downregulation in level of expression of THTR-1 and THTR-2 at the protein and mRNA levels and a decrease in transcriptional (promoter) activity. Modulators of intracellular Ca2+/calmodulin- and protein-tyrosine kinase-mediated pathways also altered thiamin uptake. Finally, confocal imaging of live beta-TC-6 cells showed that clinical mutants of THTR-1 have mixed expression phenotypes and all led to impairment in thiamin uptake. These studies demonstrate for the first time that thiamin uptake by the endocrine pancreas is carrier mediated and is adaptively regulated by the prevailing vitamin level via transcriptional mechanisms. Furthermore, clinical mutants of THTR-1 impair thiamin uptake via different mechanisms.
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