ArticleJournal of the American Society of Nephrology : JASN2009
Nucleotides downregulate aquaporin 2 via activation of apical P2 receptors.
Article in Journal of the American Society of Nephrology : JASN, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Molecular insights into P2X signalling cascades in acute kidney injury.Purinergic signalling · 2024Review
- Epac induces ryanodine receptor-dependent intracellular and inter-organellar calcium mobilization in mpkCCD cells.Frontiers in physiology · 2023Article
- A Vasopressin-Induced Change in Prostaglandin Receptor Subtype Expression Explains the Differential Effect of PGEFrontiers in physiology · 2021Article
- Purinergic signaling is enhanced in the absence of UT-A1 and UT-A3.Physiological reports · 2021Article
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- P2X6 Knockout Mice Exhibit Normal Electrolyte Homeostasis.PloS one · 2016Article
- Deep proteomic profiling of vasopressin-sensitive collecting duct cells. I. Virtual Western blots and molecular weight distributions.American journal of physiology. Cell physiology · 2015Article
- Relative roles of principal and intercalated cells in the regulation of sodium balance and blood pressure.Current hypertension reports · 2015Review
- Deciphering physiological role of the mechanosensitive TRPV4 channel in the distal nephron.American journal of physiology. Renal physiology · 2015Review
- P2X4 receptor regulation of transient receptor potential melastatin type 6 (TRPM6) Mg2+ channels.Pflugers Archiv : European journal of physiology · 2014Article
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- P2 receptors in renal autoregulation.Current vascular pharmacology · 2014Review
- Renal β-intercalated cells maintain body fluid and electrolyte balance.The Journal of clinical investigation · 2013Article
- Emerging key roles for P2X receptors in the kidney.Frontiers in physiology · 2013Article
- Cell culture models and animal models for studying the patho-physiological role of renal aquaporins.Cellular and molecular life sciences : CMLS · 2012Review
- A new look at electrolyte transport in the distal tubule.Annual review of physiology · 2012Review
- P2Y receptors and kidney function.Wiley interdisciplinary reviews. Membrane transport and signaling · 2012Article
- Regulation of renal NaCl and water transport by the ATP/UTP/P2Y2 receptor system.American journal of physiology. Renal physiology · 2011Review
- Bench-to-bedside review: Vasopressin in the management of septic shock.Critical care (London, England) · 2011Review
- Genetic analysis of mouse strains with variable serum sodium concentrations identifies the Nalcn sodium channel as a novel player in osmoregulation.Physiological genomics · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Vasopressin regulates water reabsorption in the collecting duct, but extracellular nucleotides modulate this regulation through incompletely understood mechanisms. We investigated these mechanisms using immortalized mouse collecting duct (mpkCCD) cells. Basolateral exposure to dDAVP induced AQP2 localization to the apical membrane, but co-treatment with ATP internalized AQP2. Because plasma membrane-bound P2 receptors (P2R) mediate the effects of extracellular nucleotides, we examined the abundance and localization of P2R in mpkCCD cells. In the absence of dDAVP, P2Y(1) and P2Y(4) receptors localized to the apical membrane, whereas P2X(2), P2X(4), P2X(5), P2X(7), P2Y(2), P2Y(11), and P2Y(12) receptors localized to the cytoplasm. dDAVP induced gene expression of P2X(1), which localized to the apical domain, and led to translocation of P2X(2) and P2Y(2) to the apical and basolateral membranes, respectively. In co-expression experiments, P2R activation decreased membrane AQP2 and AQP2-mediated water permeability in Xenopus oocytes expressing P2X(2), P2Y(2,) or P2Y(4) receptors, but not in oocytes expressing other P2R subtypes. In summary, these data suggest that AQP2-mediated water transport is downregulated not only by basolateral nucleotides, mediated by P2Y(2) receptors, but also by luminal nucleotides, mediated by P2X(2) and/or P2Y(4) receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.