ArticleBMC microbiology2009
Genomic analysis of Campylobacter fetus subspecies: identification of candidate virulence determinants and diagnostic assay targets.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 59 citations in OpenAlex.
- Identification of virulence genes and clade-specific markers through pan-genomic analysis of Leptospira.BMC microbiology · 2025Article
- Campylobacter fetus Plasmid Diversity: Comparative Analysis of Fully Sequenced Plasmids and Proposed Classification Scheme.Genome biology and evolution · 2024Article
- Biochemical and molecular characterization of Campylobacter fetus isolates from bulls subjected to bovine genital campylobacteriosis diagnosis in Spain.BMC veterinary research · 2024Article
- Comparative pangenomic analysis of Campylobacter fetus isolated from Spanish bulls and other mammalian species.Scientific reports · 2024Article
- Whole-genome comparison using complete genomes fromFrontiers in microbiology · 2024Article
- Bovine campylobacteriosis in bulls: insights in the conventional and molecular diagnosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Prevalence of Bovine Genital Campylobacteriosis, Associated Risk Factors and Spatial Distribution in Spanish Beef Cattle Based on Veterinary Laboratory Database Records.Frontiers in veterinary science · 2021Article
- Assessment of Campylobacter fetus subsp. venerealis molecular diagnosis using clinical samples of bulls.BMC veterinary research · 2020Article
- Phylogenomic Analysis ofFrontiers in microbiology · 2020Article
- Identification of Campylobacter fetus subsp. venerealis virulence genes in cervical mucus from cows.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Pathogenomics of EmergingClinical microbiology reviews · 2019Review
- Article
- Complete Genome Sequence of Type Strain Campylobacter fetus subsp. fetus ATCC 27374.Genome announcements · 2016Article
- A novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequences.BMC veterinary research · 2016Article
- Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids.PloS one · 2016Article
- The Cytolethal Distending Toxin Contributes to Microbial Virulence and Disease Pathogenesis by Acting As a Tri-Perditious Toxin.Frontiers in cellular and infection microbiology · 2016Review
- Inconsistency of phenotypic and genomic characteristics of Campylobacter fetus subspecies requires reevaluation of current diagnostics.Journal of clinical microbiology · 2014Article
- Draft Genome Sequences of Campylobacter fetus subsp. venerealis bv. venerealis Strain B6 and bv. intermedius Strain 642-21.Genome announcements · 2014Article
- Genomic evidence for the emergence and evolution of pathogenicity and niche preferences in the genus Campylobacter.Genome biology and evolution · 2014Article
- Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCampylobacter fetus subspecies venerealis is the causative agent of bovine genital campylobacteriosis, asymptomatic in bulls the disease is spread to female cattle causing extensive reproductive loss. The microbiological and molecular differentiation of C. fetus subsp. venerealis from C. fetus subsp. fetus is extremely difficult. This study describes the analysis of the available C. fetus subsp. venerealis AZUL-94 strain genome (approximately 75-80%) to identify elements exclusively found in C. fetus subsp. venerealis strains as potential diagnostic targets and the characterisation of subspecies virulence genes.
resultsEighty Kb of genomic sequence (22 contigs) was identified as unique to C. fetus subsp. venerealis AZUL-94 and consisted of type IV secretory pathway components, putative plasmid genes and hypothetical proteins. Of the 9 PCR assays developed to target C. fetus subsp. venerealis type IV secretion system genes, 4 of these were specific for C. fetus subsp. venerealis biovar venerealis and did not detect C. fetus subsp. venerealis biovar intermedius. Two assays were specific for C. fetus subsp. venerealis AZUL-94 strain, with a further single assay specific for the AZUL-94 strain and C. fetus subsp. venerealis biovar intermedius (and not the remaining C. fetus subsp. venerealis biovar venerealis strains tested). C. fetus subsp. fetus and C. fetus subsp. venerealis were found to share most common Campylobacter virulence factors such as SAP, chemotaxis, flagellar biosynthesis, 2-component systems and cytolethal distending toxin subunits (A, B, C). We did not however, identify in C. fetus the full complement of bacterial adherence candidates commonly found in other Campylobacter spp.
conclusionThe comparison of the available C. fetus subsp. venerealis genome sequence with the C. fetus subsp. fetus genome identified 80 kb of unique C. fetus subsp. venerealis AZUL94 sequence, with subsequent PCR confirmation demonstrating inconsistent amplification of these targets in all other C. fetus subsp. venerealis strains and biovars tested. The assays developed here highlight the complexity of targeting strain specific virulence genes for field studies for the molecular identification and epidemiology of C. fetus.
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