ArticleAmerican journal of physiology. Cell physiology2009
Transfer of ascorbic acid across the vascular endothelium: mechanism and self-regulation.
Article in American journal of physiology. Cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 36 citations in OpenAlex.
- Sodium ascorbate versus ascorbic acid in sepsis: a narrative review of emerging cardiovascular and neurological benefits.Critical care (London, England) · 2026Review
- Identification of Nanoparticle Properties for Optimal Drug Delivery across a Physiological Cell Barrier.Pharmaceutics · 2023Article
- The Effects of Vitamin C on the Multiple Pathophysiological Stages of COVID-19.Life (Basel, Switzerland) · 2021Review
- The Subjective Well-being and Health-Related Quality of Life of Australian Adults with Increased Intestinal Permeability and Associations with Treatment Interventions.Journal of alternative and complementary medicine (New York, N.Y.) · 2021Article
- Review
- Intracellular ascorbate tightens the endothelial permeability barrier through Epac1 and the tubulin cytoskeleton.American journal of physiology. Cell physiology · 2016Article
- Ascorbic acid repletion: A possible therapy for diabetic macular edema?Free radical biology & medicine · 2016Review
- Ascorbic acid prevents VEGF-induced increases in endothelial barrier permeability.Molecular and cellular biochemistry · 2016Article
- Recreating blood-brain barrier physiology and structure on chip: A novel neurovascular microfluidic bioreactor.Biomicrofluidics · 2015Article
- Intracellular Ascorbate Prevents Endothelial Barrier Permeabilization by Thrombin.The Journal of biological chemistry · 2015Article
- Vitamin C deficiency in the brain impairs cognition, increases amyloid accumulation and deposition, and oxidative stress in APP/PSEN1 and normally aging mice.ACS chemical neuroscience · 2015Article
- Identification of vitamin C transporters in the human airways: a cross-sectional in vivo study.BMJ open · 2015Article
- Vitamin C in Health and Disease: Its Role in the Metabolism of Cells and Redox State in the Brain.Frontiers in physiology · 2015Review
- Effect of temperature, pH and heme ligands on the reduction of Cygb(Fe(3+)) by ascorbate.Archives of biochemistry and biophysics · 2014Article
- Intravenous ascorbate improves spatial memory in middle-aged APP/PSEN1 and wild type mice.Behavioural brain research · 2014Article
- Ascorbate reverses high glucose- and RAGE-induced leak of the endothelial permeability barrier.Biochemical and biophysical research communications · 2014Article
- Myths, artifacts, and fatal flaws: identifying limitations and opportunities in vitamin C research.Nutrients · 2013Review
- Role of vitamin C in the function of the vascular endothelium.Antioxidants & redox signaling · 2013Review
- Ascorbate-induced generation of 5-hydroxymethylcytosine is unaffected by varying levels of iron and 2-oxoglutarate.Biochemical and biophysical research communications · 2013Article
- Differences in oxygen-dependent nitric oxide metabolism by cytoglobin and myoglobin account for their differing functional roles.The FEBS journal · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To determine how ascorbic acid moves from the bloodstream into tissues, we assessed transfer of the vitamin across the barrier generated by EA.hy926 endothelial cells when these were cultured on semipermeable filter supports. Ascorbate transfer from the luminal to the abluminal compartment was time dependent, inhibited by anion channel blockers and by activation of protein kinase A, but was increased by thrombin. Ascorbate transfer occurred by a paracellular route, since it did not correlate with intracellular ascorbate contents and was not rectified or saturable. Nonetheless, intracellular ascorbate inhibited the transfer of both ascorbate and radiolabeled inulin across the endothelial barrier. The increase in barrier function due to ascorbate was dependent on its intracellular concentration, significant by 15 min of incubation, prevented by the cytoskeletal inhibitor colchicine, associated with F-actin stress fiber formation, and not due to collagen deposition. These results show that ascorbate traverses the endothelial barrier by a paracellular route that is regulated by cell metabolism, ion channels, and ascorbate itself. Since the latter effect occurred over the physiological range of ascorbate plasma concentrations, it could reflect a role for the vitamin in control of endothelial barrier function in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.