ArticleBMC cell biology2009
Testis-expressed profilins 3 and 4 show distinct functional characteristics and localize in the acroplaxome-manchette complex in spermatids.
Article in BMC cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 32 citations in OpenAlex.
- Profilin and Its Isoforms: Unravelling the Functional Diversity Across Life Forms.Current protein & peptide science · 2026Review
- Profilin and Non-Canonical Wnt Signaling: Coordinating Cytoskeletal Dynamics from Development to Disease.Journal of developmental biology · 2025Review
- Exploring the impact of lipid stress on sperm cytoskeleton: insights and prospects.Nature reviews. Urology · 2025Review
- Testis-specific actin-like 7A (ACTL7A) is an indispensable protein for subacrosomal-associated F-actin formation, acrosomal anchoring, and male fertility.Molecular human reproduction · 2023Article
- PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis.Development (Cambridge, England) · 2022Article
- Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells.Nature communications · 2022Article
- Sperm bauplan and function and underlying processes of sperm formation and selection.Physiological reviews · 2022Review
- A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1-RhoA/Rac1.Journal of cellular and molecular medicine · 2021Article
- Loss of Profilin3 Impairs Spermiogenesis by Affecting Acrosome Biogenesis, Autophagy, Manchette Development and Mitochondrial Organization.Frontiers in cell and developmental biology · 2021Article
- Profilin Isoforms in Health and Disease - All the Same but Different.Frontiers in cell and developmental biology · 2021Review
- PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin.The Journal of biological chemistry · 2020Article
- Loss of myosin VI expression affects acrosome/acroplaxome complex morphology during mouse spermiogenesis†.Biology of reproduction · 2020Article
- Sperm Differentiation: The Role of Trafficking of Proteins.International journal of molecular sciences · 2020Review
- HIPK4 is essential for murine spermiogenesis.eLife · 2020Article
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- Expression and localization of myosin VI in developing mouse spermatids.Histochemistry and cell biology · 2017Article
- Structural, Functional, and Immunological Characterization of Profilin Panallergens Amb a 8, Art v 4, and Bet v 2.The Journal of biological chemistry · 2016Article
- Recombinant production, crystallization and preliminary structural characterization of Schistosoma japonicum profilin.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- MARCH7 E3 ubiquitin ligase is highly expressed in developing spermatids of rats and its possible involvement in head and tail formation.Histochemistry and cell biology · 2013Article
- mDia1-3 in mammalian filopodia.Communicative & integrative biology · 2012Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundMultiple profilin isoforms exist in mammals; at least four are expressed in the mammalian testis. The testis-specific isoforms profilin-3 (PFN3) and profilin-4 (PFN4) may have specialized roles in spermatogenic cells which are distinct from known functions fulfilled by the "somatic" profilins, profilin-1 (PFN1) and profilin-2 (PFN2).
resultsLigand interactions and spatial distributions of PFN3 and PFN4 were compared by biochemical, molecular and immunological methods; PFN1 and PFN2 were employed as controls. beta-actin, phosphoinositides, poly-L-proline and mDia3, but not VASP, were confirmed as in vitro interaction partners of PFN3. In parallel experiments, PFN4 bound to selected phosphoinositides but not to poly-L-proline, proline-rich proteins, or actin. Immunofluorescence microscopy of PFN3 and PFN4 revealed distinct subcellular locations in differentiating spermatids. Both were associated first with the acroplaxome and later with the transient manchette. Predicted 3D structures indicated that PFN3 has the actin-binding site conserved, but retains only approximately half of the common poly-L-proline binding site. PFN4, in comparison, has lost both, polyproline and actin binding sites completely, which is well in line with the experimental data.
conclusionThe testis-specific isoform PFN3 showed major hallmarks of the well characterized "somatic" profilin isoforms, albeit with distinct binding affinities. PFN4, on the other hand, did not interact with actin or polyproline in vitro. Rather, it seemed to be specialized for phospholipid binding, possibly providing cellular functions which are distinct from actin dynamics regulation.
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