ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2009
Oxygen tension regulates the expression of ANK (progressive ankylosis) in an HIF-1-dependent manner in growth plate chondrocytes.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Synovial Membrane Is a Major Producer of Extracellular Inorganic Pyrophosphate in Response to Hypoxia.Pharmaceuticals (Basel, Switzerland) · 2024Article
- The potential role and trend of HIF‑1α in intervertebral disc degeneration: Friend or foe? (Review).Molecular medicine reports · 2021Review
- Ultrashort Time to Echo Magnetic Resonance Evaluation of Calcium Pyrophosphate Crystal Deposition in Human Menisci.Investigative radiology · 2019Article
- Demographic History and Genetic Adaptation in the Himalayan Region Inferred from Genome-Wide SNP Genotypes of 49 Populations.Molecular biology and evolution · 2018Article
- Transcription factor HIF1A: downstream targets, associated pathways, polymorphic hypoxia response element (HRE) sites, and initiative for standardization of reporting in scientific literature.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- The Role of ANK in Calcium Pyrophosphate Deposition Disease.Current rheumatology reports · 2016Review
- Evaluation of insulin medium or chondrogenic medium on proliferation and chondrogenesis of ATDC5 cells.BioMed research international · 2014Article
- Genetics and mechanisms of crystal deposition in calcium pyrophosphate deposition disease.Current rheumatology reports · 2012Review
- Hypoxia-inducible factor regulation of ANK expression in nucleus pulposus cells: possible implications in controlling dystrophic mineralization in the intervertebral disc.Arthritis and rheumatism · 2010Article
- The inorganic pyrophosphate transporter ANK preserves the differentiated phenotype of articular chondrocyte.The Journal of biological chemistry · 2010Article
- Additive and synergistic effects of bFGF and hypoxia on leporine meniscus cell-seeded PLLA scaffolds.Journal of tissue engineering and regenerative medicine · 2010Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The proximal promoter region of ANK, a gene that codes for a protein that regulates the transport of inorganic pyrophosphate, contains two hypoxia responsive elements (HREs); therefore, we studied the expression and function of ANK at different oxygen tensions. ATDC5 and N1511 clonal chondrocytic cells were cultured in either hypoxia (2% O(2)) or normoxia (21% O(2)). Transcript and protein levels of ANK were depressed in hypoxic conditions, as were levels of extracellular pyrophosphate (ePPi). To determine whether HIF-1 was involved in the oxemic response, Hif-1alpha knockdown cells were exposed to varying oxygen conditions and ANK expression was assessed. Knockdown of Hif-1alpha resulted in low levels of expression of ANK in hypoxia and normoxia. Chromatin immunoprecipitation (ChIP) assays explored the binding of Hif-1alpha to ANK HREs and showed that Hif-1alpha is able to bind to the HREs of ANK more avidly in normoxia than in hypoxia. Furthermore, functional studies of Hif-1alpha activity using luciferase reporter assays of wildtype and mutagenized HREs showed that only HRE-1 binds Hif-1alpha in normoxia. Expression of ANK in growth plate and articular cartilage was low in hypoxic regions of the tissues, and higher levels of ANK expression were observed in the synovium and meniscus in regions that have a normally higher oxygen tension. The data suggest that ANK expression and function in vitro and in vivo are repressed in hypoxic environments and that the effect is regulated by HIF-1.
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