ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2009
Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 49 citations in OpenAlex.
- Diagnostic Use of CCR3, CD63, CD203c and FcεRIα on Blood Leukocytes of Allergic Asthma and Combined Allergic Rhinitis and Asthma Syndrome.Journal of cellular and molecular medicine · 2025Article
- Elevated Basal Serum Tryptase: Disease Distribution and Variability in a Regional Health System.The journal of allergy and clinical immunology. In practice · 2022Article
- The Clinical and Biological Effects of Homeopathically Prepared Signaling Molecules: A Scoping Review.Homeopathy : the journal of the Faculty of Homeopathy · 2022Article
- Does homeopathic medicine have a preventive effect on respiratory tract infections? A real life observational study.Multidisciplinary respiratory medicine · 2016Article
- Mendelian inheritance of elevated serum tryptase associated with atopy and connective tissue abnormalities.The Journal of allergy and clinical immunology · 2014Article
- Plausibility and evidence: the case of homeopathy.Medicine, health care, and philosophy · 2013Article
- Homeopathic Doses of Gelsemium sempervirens Improve the Behavior of Mice in Response to Novel Environments.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice.Psychopharmacology · 2010Article
- Reporting experiments in homeopathic basic research (REHBaR)--a detailed guideline for authors.Homeopathy : the journal of the Faculty of Homeopathy · 2009Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivePrevious research suggests that human basophil activation may be inhibited by histamine even at extremely low doses (high dilutions). However, uncertainties about the nature of the phenomenon and its reproducibility mean that further, rigorously controlled studies are necessary.
methodsSerial 1:100 (v:v) histamine dilutions (centesimal dilutions, C) and water controls were tested on human basophil responsiveness to anti-IgE antibodies, using flow cytometry. Each dilution step was followed by vertical mechanical shaking (also designed as succussion) at 20 strokes/s. Basophil-enriched buffy coats from healthy blood donors were incubated with 10(-4) mol/l histamine (2C) and with serially diluted preparations from 10(-20) mol/l (10C) to 10(-32) mol/l (16C), then incubated for 30 min with 1 mug/ml goat monoclonal anti-human IgE and basophils stained for immunophenotyping.
resultsMembrane up-regulation of CD203c, which in these experimental conditions proved to be a more consistent activation marker than CD63, was significantly inhibited in samples treated with histamine at the dilutions of 2C (P = 0.001), 12C (P = 0.047), 14C (P = 0.003), 15C (P = 0.036) and 16C (P = 0.009). Control water dilutions/succussions did not show any significant effect.
conclusionUsing a strictly standardized flow cytometry protocol and a new dilution/succussion procedure, we have shown that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils.
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