ArticleLab on a chip2009
Enhancing the performance of a point-of-care CD4+ T-cell counting microchip through monocyte depletion for HIV/AIDS diagnostics.
Article in Lab on a chip, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- The potential of microfluidic cell analysis in CAR T cell therapy manufacturing.Frontiers in bioengineering and biotechnology · 2025Review
- Visualization and Analysis of Mapping Knowledge Domain of Fluid Flow Related to Microfluidic Chip.ACS omega · 2024Article
- Extending the Shelf-Life of Immunoassay-Based Microfluidic Chips through Freeze-Drying Sublimation Techniques.Sensors (Basel, Switzerland) · 2023Article
- Advances in Continuous Microfluidics-Based Technologies for the Study of HIV Infection.Viruses · 2020Review
- Rapid, multiplexed detection of biomolecules using electrically distinct hydrogel beads.Lab on a chip · 2020Article
- An immunoassay cassette with a handheld reader for HIV urine testing in point-of-care diagnostics.Biomedical microdevices · 2020Article
- Point of care technologies for sepsis diagnosis and treatment.Lab on a chip · 2019Review
- Cell Cytometry: Review and Perspective on Biotechnological Advances.Frontiers in bioengineering and biotechnology · 2019Review
- Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells.Bioengineering (Basel, Switzerland) · 2017Article
- A point-of-care microfluidic biochip for quantification of CD64 expression from whole blood for sepsis stratification.Nature communications · 2017Article
- Innovative Programmable Bio-Nano-Chip Digitizes Biology Using Sensors That Learn Bridging Biomarker Discovery and Clinical Implementation.Frontiers in public health · 2017Review
- Article
- Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring.Advanced drug delivery reviews · 2016Review
- Microfluidic differential immunocapture biochip for specific leukocyte counting.Nature protocols · 2016Article
- Application of programmable bio-nano-chip system for the quantitative detection of drugs of abuse in oral fluids.Drug and alcohol dependence · 2015Article
- Reconfigurable microfluidic device with integrated antibody arrays for capture, multiplexed stimulation, and cytokine profiling of human monocytes.Biomicrofluidics · 2015Article
- Exclusion-Based Capture and Enumeration of CD4+ T Cells from Whole Blood for Low-Resource Settings.Journal of laboratory automation · 2014Article
- A Biochip with a 3D microfluidic architecture for trapping white blood cells.Sensors and actuators. B, Chemical · 2013Article
- Manipulating biological agents and cells in micro-scale volumes for applications in medicine.Chemical Society reviews · 2013Review
- p38 signaling and receptor recycling events in a microfluidic endothelial cell adhesion assay.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
CD4+ T cell counts are important tests used to stage HIV-positive patients, enabling clinicians to make informed antiretroviral treatment decisions and to monitor the therapeutic outcomes. However, state-of-the-art CD4 counting methods based on flow cytometry are not applicable in resource-limited settings, due to their high cost and technical requirements. In previous work, we reported the development of a cell isolation microchip that can be used at the point of care for CD4 counts. In that microfluidic chip, CD4+ T cells were separated from 10 microL of whole blood, and enumerated via either light microscopy or impedance sensing. The microchip counts matched flow cytometry results in the intermediate CD4 count range, between 200-800 cells/microL, but displayed a positive bias at absolute CD4 counts below 200 cells/microL, due largely to monocyte contamination. To enhance the performance in the low CD4 count range, we report here an improved design of a two-stage microfluidic device to deplete monocytes from whole blood, followed by CD4+ T cell capture. Using the double-stage device combined with a high viscosity rinsing solution, we obtained microchip CD4 counts comparable to flow cytometry results in the full clinically relevant range. In addition to CD4 counting, the strategy of contaminant depletion prior to target cell isolation can be easily adapted to immunoaffinity capture of other cell types that lack a unique surface marker from a complex biological fluid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.